Abstract 1222: Simultaneous detection of 30 cytokines in human sera using barcoded magnetic bead technology.

Abstract

Cytokines mediate a wide range of physiological responses including immunity, inflammation, and hematopoiesis, which are important circulating proteins. It has been well demonstrated that cytokines are associated with a spectrum of diseases ranging from tumor growth to infection and to Parkinson. Traditionally, cytokines are measured by immunoblot or immunoassay, which are both time consuming and not cost effective. There is an increasing need for multiplexing assays in this field. With the development of bead array technology, which uses fluorescent color-coated microspheres analyzed with a dedicated reader, simultaneous detection of multiple analytes becomes possible in a single well of a microtiter plate. Although the bead array technology becomes more and more popular and dominated in the multiplexing assay market, there are still some limitations and potential issues from this technology, such as photobleaching of the fluorescent color-coated beads and expensive laser detector. Recently, a new generation of the reader has been developed, which replaces laser detector with CCD camera. However, it still uses fluorescent color-coated beads, which makes the assay sensitive to light and unstable. Here we present a new Barcoded Magnetic Bead technology, which employs a permanent bar code on magnetic beads with high resolution CCD camera for scanning. This technology has successfully solved the problems from fluorescent color-coated bead and makes the multiplex assay much more stable and reliable. We can detect 30 cytokines from human sera with limit of detection less than 5 pg/ml, while inter- and intra assay CV less than 10%. The results are correlated very well with the bead array technology and traditional ELISA. In addition, our multiplex protein assays have unique internal control in each sample well, which makes the assay much more reliable and robust. This multiplex technology can be used in both life science research and clinical fields.

Citation Format: Aiguo Zhang, Rachel Chuang, Lily Chen, XiNing Zhu, Rachel Diaz, Dean Tsao. Simultaneous detection of 30 cytokines in human sera using barcoded magnetic bead technology. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 1222. doi:10.1158/1538-7445.AM2013-1222