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Molecular and Cellular Biology

Abstract 4220: Establishment of a rapid and automated detection system for BRAF mutations in malignant melanoma.

Masaru Ide, Shinichi Koba, Yumi Nagano, Naoko Aragane-Sueoka, Akemi Sato, Takuya Inoue, Naomi Kobayashi, Noriyuki Misago, Yutaka Narisawa, Shinya Kimura and Eisaburo Sueoka
Masaru Ide
Saga Univ., Saga, Japan.
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Shinichi Koba
Saga Univ., Saga, Japan.
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Yumi Nagano
Saga Univ., Saga, Japan.
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Naoko Aragane-Sueoka
Saga Univ., Saga, Japan.
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Akemi Sato
Saga Univ., Saga, Japan.
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Takuya Inoue
Saga Univ., Saga, Japan.
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Naomi Kobayashi
Saga Univ., Saga, Japan.
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Noriyuki Misago
Saga Univ., Saga, Japan.
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Yutaka Narisawa
Saga Univ., Saga, Japan.
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Shinya Kimura
Saga Univ., Saga, Japan.
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Eisaburo Sueoka
Saga Univ., Saga, Japan.
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DOI: 10.1158/1538-7445.AM2013-4220 Published April 2013
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Proceedings: AACR 104th Annual Meeting 2013; Apr 6-10, 2013; Washington, DC

Abstract

BRAF gene mutations have been observed in 30-70% of malignant melanoma patients. Recent development of therapeutic intervention using BRAF inhibitors requires an accurate and rapid detection system for BRAF mutations. In addition, there are only a few reports that investigated the clinical characteristics of the melanoma associated with BRAF mutations in Japanese patients on a large scale evaluation. We recently established quenching probe system (QP) for detection of an activating BRAF mutation, V600E and evaluated 113 melanoma samples diagnosed in Saga University Hospital from 1982 to 2011. The QP system includes fully automated genotyping, based on analysis of the probe DNA melting curve, which binds the target mutated site using a fluorescent guanine quenched probe. BRAF mutations were detected in 54 of 115 (47%) including 51 of V600E and 3 of V600K in Japanese melanoma cases. Among clinical subtypes of melanoma, nodular melanoma showed high frequency (12 of 15; 80%) of mutation followed by superficial spreading melanoma (13 of 26; 50%). The QP system is a simple and sensitive method to determine BRAF V600E mutation, and will be useful tool for patient-oriented therapy with BRAF inhibitors.

Citation Format: Masaru Ide, Shinichi Koba, Yumi Nagano, Naoko Aragane-Sueoka, Akemi Sato, Takuya Inoue, Naomi Kobayashi, Noriyuki Misago, Yutaka Narisawa, Shinya Kimura, Eisaburo Sueoka. Establishment of a rapid and automated detection system for BRAF mutations in malignant melanoma. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 4220. doi:10.1158/1538-7445.AM2013-4220

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Cancer Research: 73 (8 Supplement)
April 2013
Volume 73, Issue 8 Supplement
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Abstract 4220: Establishment of a rapid and automated detection system for BRAF mutations in malignant melanoma.
Masaru Ide, Shinichi Koba, Yumi Nagano, Naoko Aragane-Sueoka, Akemi Sato, Takuya Inoue, Naomi Kobayashi, Noriyuki Misago, Yutaka Narisawa, Shinya Kimura and Eisaburo Sueoka
Cancer Res April 15 2013 (73) (8 Supplement) 4220; DOI: 10.1158/1538-7445.AM2013-4220

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Abstract 4220: Establishment of a rapid and automated detection system for BRAF mutations in malignant melanoma.
Masaru Ide, Shinichi Koba, Yumi Nagano, Naoko Aragane-Sueoka, Akemi Sato, Takuya Inoue, Naomi Kobayashi, Noriyuki Misago, Yutaka Narisawa, Shinya Kimura and Eisaburo Sueoka
Cancer Res April 15 2013 (73) (8 Supplement) 4220; DOI: 10.1158/1538-7445.AM2013-4220
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Cancer Research Online ISSN: 1538-7445
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