Abstract 2518: Effective vaccination against melanoma in an animal study: Combination of laser-assisted dermal skin delivery and cross-presenting XCR1+ dermal DCs targeting

Abstract

Background/Aim/Method

The induction of CD8⁺ cytotoxic T lymphocytes (CTL) is critical to eradicate tumor cells. In mouse skin, a subset of dendritic cell characterised by the expression of the chemokine receptor XCR1 is highly potent to activate CD8⁺ T cells. By combining PLEASE-assisted laser poration of the skin allowing dermal delivery and the use of dimeric vaccine molecule in which the XCR1 ligand, Xcl1, was fused to the antigen, we targeted specifically this XCR1⁺ dermal subset.

Results

Dermal delivery of this vaccine molecule after skin laser-poration induced specific strong T cell proliferation in an XCR1-dependent manner. A single immunization allowed protecting against melanoma in both therapeutic and prophylactic protocols.

Discussion & Conclusion

Thus, specific targeting of cross-presenting skin DCs represents a promising vaccine strategy for induction of CD8⁺ T cell responses and protection against cancers.

Citation Format: Dorothea Terhorst, Even Fossum, Anna Baranska, Samira Tamoutounour, Camille Malosse, Mattia Garbani, Elmira Lechat, Reto Crameri, Roland Winteler, Bjarne Bogen, Bernard Malissen, Sandrine Henri. Effective vaccination against melanoma in an animal study: Combination of laser-assisted dermal skin delivery and cross-presenting XCR1+ dermal DCs targeting. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 2518. doi:10.1158/1538-7445.AM2015-2518