Background: Vascular endothelial growth factor A (VEGFA) has pivotal roles in angiogenesis, vascular permeability, vasculogenesis, cellular growth, and cell migration. We investigated whether single nucleotide polymorphisms (SNPs) of the VEGFA gene are associated with the development of papillary thyroid cancer (PTC). We also assessed the relationships between VEGFA SNPs and the clinicopathologic characteristics such as the size of cancers (< 1 cm and ≥ 1 cm), numbers of cancers (unifocality and multifocality), location of cancers (one lobe and both lobe), extrathyroidal invasion (present and absent), and cervical lymph node metastasis (present and absent).
Methods: One promoter SNP (rs699947, -2578A/C) and one 5′-untranslation region (5′UTR) SNP (rs2010963, +405C/G) in VEGFA were genotyped using direct sequencing in 94 PTC patients and 213 healthy controls. SNPStats, SNPAnalyzer, Helixtree, and Haploview version 4.2 were used to analyze genetic data. Multiple logistic regression models (codominant, dominant, recessive, and log-additive) were performed to evaluate odds ratios (ORs), 95% confidence intervals (CIs), and p values.
Results: Two SNPs of VEGFA were not associated with the development of PTC. Also, haplotypes were not associated with PTC??. However, we found a relationship between the 5′UTR SNP rs2010963 and PTC with the extrathyroidal invasion (p = 0.0060 in codominant model, p = 0.0258 in dominant model, p = 0.0057 in recessive model, and p = 0.0060 in log-additive model). Allele frequencies of rs2010963 were different between the extrathyroidal invasion (-) and extrathyroidal invasion (+) groups (p = 0.0050). In Bonferroni correction, these correlations were also significant except the recessive model.
Conclusion: Our results suggest that the 5′UTR SNP rs2010963 (+405C/G) of VEGFA may be associated with susceptibility to extrathyroidal invasion of PTC in Korean population.
Note: This abstract was not presented at the meeting.
Citation Format: Young Gyu Eun, Young Chan Lee, Jung-Woo Lee. A polymorphism of VEGFA is associated with susceptibility to extrathyroidal invasion of papillary thyroid cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 5280. doi:10.1158/1538-7445.AM2015-5280
- ©2015 American Association for Cancer Research.