Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Molecular and Cellular Biology, Genetics

Abstract 1467: A large set of miRNAs is deregulated since the earliest steps of human HCC

Francesca Rizzo, Luca Di Tommaso, Pia Sulas, Elena Coviello, Chiara Novello, Alessandro Weisz, Massimo Roncalli and Amedeo Columbano
Francesca Rizzo
University of Salerno, Salerno, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Luca Di Tommaso
Humanitas University, Rozzano, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pia Sulas
University of Cagliari, Cagliari, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elena Coviello
University of Salerno, Salerno, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chiara Novello
Humanitas University, Rozzano, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alessandro Weisz
University of Salerno, Salerno, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Massimo Roncalli
Humanitas University, Rozzano, Italy;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amedeo Columbano
University of Cagliari, Cagliari, Italy.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.AM2017-1467 Published July 2017
  • Article
  • Info & Metrics
Loading
Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC

Abstract

Introduction: Hepatocellular carcinoma (HCC) is the result of a stepwise process preceded by the development of premalignant lesions. To assess the involvement of microRNAs (miRNAs) in hepatocarcinogenesis it is critical to understand whether and which miRNAs are involved in the early stages of tumor development. To this end, changes in expression of miRNAs were investigated in low and high grade dysplastic nodules (LGDNs, HGDNs), early HCCs (eHCCs) and progressed HCCs (pHCCs).

Materials and Methods: Small RNA sequencing (smallRNA-Seq) was applied to search for liver miRNAs and to profile their expression patterns in 14 cirrhotic nodules (CNs), 9 LGDNs, 6 HGDNs, 6 eHCCs and 20 pHCCs) from 17 patients with liver lesions of different etiology (HCV, HBV and alcohol). To investigate miRNAs differentially expressed in pHCCs towards their matched cirrhotic tissue, we applied the Random-Variance Model (F-test) and Multivariate Permutation Test (Fold Change, FC ≥ 2.0). QRT-PCR analysis was also performed on 14 additional patients with the same etiology of the first cohort for validation of the smallRNA-Seq results.

Results: We identified a 62 miRNA expression signature that differentiates pHCCs from matched CNs. Notably, 52/62 miRNAs dysregulated in pHCCs were likewise altered in LGDN and HGDN, suggesting their possible role in HCC onset. Indeed, 28 miRNAs showed lower expression in CN that was significantly increased in LGDNs and remained high up to pHCCs. In contrast, 24 miRNAs displayed an opposite behavior, as their expression was very low or absent all throughout the tumorigenic process, compared to CNs. Interestingly, 2 miRNAs (let-7a-5p, miR-101-3p) showed a progressive decrease, suggesting that they are involved in later stages of the process. To further support the finding that dysregulation of miRNAs is a very early event in HCC development, we performed qRT-PCR in a series of LGDN, HGDN, eHCC and pHCCs from 14 additional patients. We selected for this analysis a handful of miRNAs found dysregulated by NGS analysis. The results demonstrate that all the 6 miRNAs examined (miR-429, miR-141-3p, miR-200a-3p and miR-200b-3p, miR-375 and miR-21-5p), were dysregulated at all stages of the tumorigenic process as compared to CNs.

Conclusion: The results demonstrate that miRNAs deregulation may be a critical player in human hepatocarcinogenesis, from early stages to pHCC. They also suggest that some miRNAs might represent biomarkers useful for differential diagnosis of dysplastic and neoplastic liver lesions.

Note: This abstract was not presented at the meeting.

Citation Format: Francesca Rizzo, Luca Di Tommaso, Pia Sulas, Elena Coviello, Chiara Novello, Alessandro Weisz, Massimo Roncalli, Amedeo Columbano. A large set of miRNAs is deregulated since the earliest steps of human HCC [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 1467. doi:10.1158/1538-7445.AM2017-1467

  • ©2017 American Association for Cancer Research.
Previous
Back to top
Cancer Research: 77 (13 Supplement)
July 2017
Volume 77, Issue 13 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract 1467: A large set of miRNAs is deregulated since the earliest steps of human HCC
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Abstract 1467: A large set of miRNAs is deregulated since the earliest steps of human HCC
Francesca Rizzo, Luca Di Tommaso, Pia Sulas, Elena Coviello, Chiara Novello, Alessandro Weisz, Massimo Roncalli and Amedeo Columbano
Cancer Res July 1 2017 (77) (13 Supplement) 1467; DOI: 10.1158/1538-7445.AM2017-1467

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract 1467: A large set of miRNAs is deregulated since the earliest steps of human HCC
Francesca Rizzo, Luca Di Tommaso, Pia Sulas, Elena Coviello, Chiara Novello, Alessandro Weisz, Massimo Roncalli and Amedeo Columbano
Cancer Res July 1 2017 (77) (13 Supplement) 1467; DOI: 10.1158/1538-7445.AM2017-1467
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Molecular and Cellular Biology, Genetics

  • Abstract LB-323: Comparative miRNA microarray profiling indicates miR-363 promotes chemoresistance in ovarian cancer cells by targeting the Hippo member, LATS2
  • Abstract LB-268: Rate of temporal citrate efflux from malignant mitochondria predicts clinical aggressiveness in breast tumors
  • Abstract LB-324: Methylation-associated silencing of miR-193a-3p promotes ovarian cancer aggressiveness via targeting GRB7
Show more 3

Poster Presentations - Proffered Abstracts

  • Abstract LB-328: Mir-520b as a novel molecular target for suppressing stemness phenotype of head-neck cancer by inhibiting CD44
  • Abstract LB-331: Advanced DNA-gold nanoprobe-based direct and sensitive quantification of novel microRNAs in prostate cancer
  • Abstract LB-336: Specific serine phosphorylation of IRE-1 controls enhanced splicing of XBP-1 and regulated IRE-1-dependent decay (RIDD)
Show more 3

Poster Presentations - MicroRNA Regulation of Cancer Biology 2

  • Abstract 1473: miR-1207-3p regulates c-Myc in aggressive prostate cancer
  • Abstract 1470: Fine-tuning the expression of heterogeneous network of genes involved in androgen signaling, aerobic glycolysis, apoptosis and epithelial-mesenchymal transition by microRNA-644a in prostate cancer potentiation of AR signaling therapy by miR-644a: Selective manipulation of the prostate cancer transcriptome by miR-644a
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement