Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Cancer Chemistry

Abstract 2177: The olive biophenol oleuropein selectively induces apoptosis in pancreatic cancer cells in vitro

Chloe D. Goldsmith, Helen Jankowski, Danielle Bond, Judith Weidenhofer, Costas Stathopoulos, Paul Roach and Christopher Scarlett
Chloe D. Goldsmith
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helen Jankowski
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Danielle Bond
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Judith Weidenhofer
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Costas Stathopoulos
University of Abertay, Dundee, United Kingdom.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paul Roach
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher Scarlett
The University of Newcastle, Ourimbah, Australia;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.AM2017-2177 Published July 2017
  • Article
  • Info & Metrics
Loading
Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC

Abstract

Pancreatic cancer is a devastating disease with a 5-year survival rate of less than 5%. Resistance to conventional treatment options and toxicity of current chemotherapy agents (gemcitabine) makes pancreatic cancer a target for the development of novel therapeutic agents. Oleuropein is the most abundant biophenol found in olive products; it has anti-atherogenic and anti-inflammatory properties as well as activity against cancers of the breast, colon and prostate. However, there has yet to be any investigation into the effects of oleuropein on pancreatic cancer cells. Consequently, this study aimed to assess the anti-pancreatic cancer activity of oleuropein in vitro. Two cell lines were investigated; a pancreatic cancer cell line (MiaPaCa-2) and a normal pancreas cell line (HPDE). The viability of cells after treatment with 0-200μM oleuropein was assessed using the Dojindo CCK-8 viability assay and compared to gemcitabine. The induction of apoptosis was measured by way of caspase 3/7 activation, using a MUSE flow cell analyser, and expression of Bcl-2, Bax and Bac using Western blot. Cell cycle analysis was conducted using the MUSE flow cell analyser. RNA expression was assessed using Affymetrix GeneChip® Whole Transcript (WT) expression arrays. The IC50 values for oleuropein against MiaPaCa-2 cells was148μM. However, importantly, oleuropein did not decrease the viability of HPDE cells within the treatment range. In comparision, 20nM of gemcitabine did not show selectivity; it reduced the viability of MiaPaCa-2 cells to 21% and of HPDE cells to 2%. An increase in the expression of caspase 3/7 was seen in MiaPaCa-2 cells when treated with oleuropein but it had no effect on the HPDE cells. Furthermore, when treated with oleuropein, an increase in the expression of genes involved in the NRF-2 (oxidative stress) pathway was observed in MiaPaCa-2 cells, an effect not observed in HPDE cells.

Conclusion Oleuropein selectively induced apoptosis in the pancreatic cancer cells (MiaPaCa-2), appearing non-toxic to normal pancreas cells (HPDE) within the treatment ranges; this is significant, since gemcitabine was comparatively more toxic to HPDE cells. Furthermore, the link between oleuropein and the NRF-2 pathway in MiaPaCa-2 cells justifies further study into the mechanisms of action of oleuropein and its potential as a novel therapeutic approach for pancreatic cancer.

Note: This abstract was not presented at the meeting.

Citation Format: Chloe D. Goldsmith, Helen Jankowski, Danielle Bond, Judith Weidenhofer, Costas Stathopoulos, Paul Roach, Christopher Scarlett. The olive biophenol oleuropein selectively induces apoptosis in pancreatic cancer cells in vitro [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 2177. doi:10.1158/1538-7445.AM2017-2177

  • ©2017 American Association for Cancer Research.
Previous
Back to top
Cancer Research: 77 (13 Supplement)
July 2017
Volume 77, Issue 13 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract 2177: The olive biophenol oleuropein selectively induces apoptosis in pancreatic cancer cells in vitro
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Abstract 2177: The olive biophenol oleuropein selectively induces apoptosis in pancreatic cancer cells in vitro
Chloe D. Goldsmith, Helen Jankowski, Danielle Bond, Judith Weidenhofer, Costas Stathopoulos, Paul Roach and Christopher Scarlett
Cancer Res July 1 2017 (77) (13 Supplement) 2177; DOI: 10.1158/1538-7445.AM2017-2177

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract 2177: The olive biophenol oleuropein selectively induces apoptosis in pancreatic cancer cells in vitro
Chloe D. Goldsmith, Helen Jankowski, Danielle Bond, Judith Weidenhofer, Costas Stathopoulos, Paul Roach and Christopher Scarlett
Cancer Res July 1 2017 (77) (13 Supplement) 2177; DOI: 10.1158/1538-7445.AM2017-2177
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Cancer Chemistry

  • Abstract SY01-04: High-end proteomics enables identification of breast cancer therapy resistance predictive proteins
  • Abstract SY18-02: Targeting transcriptional regulators for simultaneous modulation of p53 and NF-κB in cancer treatment
  • Abstract SY11-03: Turning enzymes on with small molecules
Show more 3

Poster Presentations - Proffered Abstracts

  • Abstract SY01-04: High-end proteomics enables identification of breast cancer therapy resistance predictive proteins
  • Abstract SY18-02: Targeting transcriptional regulators for simultaneous modulation of p53 and NF-κB in cancer treatment
  • Abstract SY11-03: Turning enzymes on with small molecules
Show more 3

Poster Presentations - Targeting p53, Apoptosis, and the Cell Cycle

  • Abstract 2168: Nucleic acid clamp-mediated recognition and transcriptional modulation of MYC oncogenes through the stabilization of G-quadruplexes
  • Abstract 2178: Growth inhibition of the crude extracts of Musa basjoo in human colon carcinoma cells
  • Abstract 2152: Protein phosphatase Mg2+ and Mn2+ dependent 1F promotes smoking-induced breast cancer by inactivating phosphorylated p53-induced signals
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement