Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Molecular and Cellular Pathobiology

miR-181a-5p Inhibits Cancer Cell Migration and Angiogenesis via Downregulation of Matrix Metalloproteinase-14

Yiyi Li, Cem Kuscu, Anna Banach, Qian Zhang, Ashleigh Pulkoski-Gross, Deborah Kim, Jingxuan Liu, Eric Roth, Ellen Li, Kenneth R. Shroyer, Paula I. Denoya, Xiaoxia Zhu, Longhua Chen and Jian Cao
Yiyi Li
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cem Kuscu
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Banach
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Qian Zhang
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ashleigh Pulkoski-Gross
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Deborah Kim
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jingxuan Liu
Department of Pathology, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric Roth
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ellen Li
Department of Medicine/Division of Gastroenterology and Hepatology, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kenneth R. Shroyer
Department of Pathology, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paula I. Denoya
Department of Surgery, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoxia Zhu
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jian.cao@sunysb.eduzhuxx01@126.comchenlonghuafl01@126.com
Longhua Chen
Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jian.cao@sunysb.eduzhuxx01@126.comchenlonghuafl01@126.com
Jian Cao
Department of Medicine/Cancer Prevention, Stony Brook University, Stony Brook, New York.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jian.cao@sunysb.eduzhuxx01@126.comchenlonghuafl01@126.com
DOI: 10.1158/0008-5472.CAN-14-2875
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Upregulation of matrix metalloproteinase MMP-14 (MT1-MMP) is associated with poor prognosis in cancer patients, but it is unclear how MMP-14 becomes elevated in tumors. Here, we show that miR-181a-5p is downregulated in aggressive human breast and colon cancers where its levels correlate inversely with MMP-14 expression. In clinical specimens, enhanced expression of MMP-14 was observed in cancer cells located at the invasive front of tumors where miR-181a-5p was downregulated relative to adjacent normal cells. Bioinformatics analyses defined a potential miR-181a-5p response element within the 3′-untranslated region of MMP-14 that was validated in reporter gene experiments. Ectopic miR-181a-5p reduced MMP-14 expression, whereas miR-181a-5p attenuation elevated MMP-14 expression. In support of a critical relationship between these two genes, miR-181a-5p–mediated reduction of MMP-14 levels was sufficient to decrease cancer cell migration, invasion, and activation of pro-MMP-2. Furthermore, this reduction in MMP-14 levels was sufficient to reduce in vivo invasion and angiogenesis in chick chorioallantoic membrane assays. Taken together, our results establish the regulation of MMP-14 in cancers by miR-181a-5p through a posttranscriptional mechanism, and they further suggest strategies to elevate miR-181a-5p to prevent cancer metastasis. Cancer Res; 75(13); 1–12. ©2015 AACR.

Footnotes

  • Note: Supplementary data for this article are available at Cancer Research Online (http://cancerres.aacrjournals.org/).

  • Received September 29, 2014.
  • Revision received February 20, 2015.
  • Accepted March 10, 2015.
  • ©2015 American Association for Cancer Research.
Next
Back to top

Published OnlineFirst June 16, 2015
doi: 10.1158/0008-5472.CAN-14-2875

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
miR-181a-5p Inhibits Cancer Cell Migration and Angiogenesis via Downregulation of Matrix Metalloproteinase-14
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
miR-181a-5p Inhibits Cancer Cell Migration and Angiogenesis via Downregulation of Matrix Metalloproteinase-14
Yiyi Li, Cem Kuscu, Anna Banach, Qian Zhang, Ashleigh Pulkoski-Gross, Deborah Kim, Jingxuan Liu, Eric Roth, Ellen Li, Kenneth R. Shroyer, Paula I. Denoya, Xiaoxia Zhu, Longhua Chen and Jian Cao
Cancer Res June 16 2015 DOI: 10.1158/0008-5472.CAN-14-2875

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
miR-181a-5p Inhibits Cancer Cell Migration and Angiogenesis via Downregulation of Matrix Metalloproteinase-14
Yiyi Li, Cem Kuscu, Anna Banach, Qian Zhang, Ashleigh Pulkoski-Gross, Deborah Kim, Jingxuan Liu, Eric Roth, Ellen Li, Kenneth R. Shroyer, Paula I. Denoya, Xiaoxia Zhu, Longhua Chen and Jian Cao
Cancer Res June 16 2015 DOI: 10.1158/0008-5472.CAN-14-2875
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • TRβ2 Bypasses Rb-Dependent Proliferation in RB1−/− Cells
  • DHHC3 Regulates Breast Tumor Growth In Vivo
  • Role of Fibroblasts in Pathogenesis of Fibroids
Show more Molecular and Cellular Pathobiology
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement