The human pancreatic tumor cell line, BxPC-3, was engineered to stably express high-levels of the Aequorea victoria green fluorescent protein (GFP). The GFP-expressing pancreatic tumor cell line was surgically orthotopically implanted (SOI) as tissue fragments in the body of the pancreas of nude mice. Intravital imaging was used for quantification of growth of micrometastasis on the liver. The diameters of the three micrometastases were 288 µm and 208 µm on the right lobe and 344 µm on the left lobe as quantified by image analysis at day-70 post SOI. For details, see the article by Bouvet et al. on p. 1534 of this issue.