PT  - JOURNAL ARTICLE
AU  - Ju, Jihyeung
AU  - Hao, Xingpei
AU  - Lee, Mao-Jung
AU  - Lu, Gang
AU  - Lambert, Joshua
AU  - Sang, Shengmin
AU  - Xiao, Hang
AU  - Newmark, Harold
AU  - Yang, Chung
TI  - γ-Tocopherol-rich mixed tocopherols (γ-TmT) inhibit colon inflammation and carcinogenesis in azoxymethane (AOM) and dextran sulfate sodium (DSS)-treated mice
DP  - 2008 May 01
TA  - Cancer Research
PG  - LB-321--LB-321
VI  - 68
IP  - 9 Supplement
4099  - http://cancerres.aacrjournals.org/content/68/9_Supplement/LB-321.short
4100  - http://cancerres.aacrjournals.org/content/68/9_Supplement/LB-321.full
SO  - Cancer Res2008 May 01; 68
AB  - AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA LB-321 We investigated the effects of γ-TmT (containing 58% γ-T, 21% δ-T, and 12% α-T) and pure γ-tocopherol on colon carcinogenesis in AOM/DSS-treated mice. Male CF-1 mice at 6 weeks of age were given a dose of AOM (10 mg/kg body weight, i.p.) and one week later, 1.5% DSS in drinking fluid for 1 week. The mice were maintained on either a γ-TmT (0.3%) or AIN93M diet, starting 1 week prior to the AOM injection, until the termination of experiment. In the AOM/DSS-treated mice, dietary γ-TmT treatment resulted in a significantly lower colon inflammation index (52% of the control) on Day 7 and number of colon adenomas (9% of the control) on Week 7. γ-TmT treatment also resulted in a higher apoptotic index in adenomas, lower prostaglandin E2, leukotriene B4, and nitrotyrosine levels in the colon, and lower prostaglandin E2, leukotriene B4, and 8-isoprotane levels in the plasma on Week 7. Some of the decreases were observed even on Day 7. γ-TmT treatment significantly increased γ-T and δ-T levels in the plasma and colon, but not significantly affecting the α-T levels. In another experiment with AOM/DSS-treated mice sacrificed at Week 21, dietary treatment with 0.17% or 0.3% γ-TmT significantly inhibited colon adenocarcinoma and adenoma formation (4-33% of the control), but the treatment with pure γ-T (0.17% in diet) did not. The present study demonstrated that γ-TmT, but not pure γ-T, significantly inhibited colon carcinogenesis in AOM/DSS-treated mice, and the inhibition may be associated with apoptosis-inducing, anti-inflammatory, antioxidative, and NOx-trapping activities.