Table 1

Antibodies used for immunohistochemistry profile, blocking, and detection kits

AntibodyClassDilutionSourceAntigen retrievalBlockingDetection kit
p53 (DO7)MMa1:200Novocastra Laboratories (Newcastle-Upon-Tyne, United Kingdom)PCLSAB+
p21MM1:8000Santa Cruz Biotechnology (Santa Cruz, CA)PCPBALSAB+
Cyclin D1RP1:400Upstate Biotechnology (Lake Placid, NY)PCLSAB+
bcl-2MM1:50DAKO Corporation (Carpinteria, CA)PCLSAB2
Ki-67RP1:100DAKO CorporationPCLSAB2
PCNAMM1:400DAKO CorporationNoneLSAB2
c-erbB-2RP1:400DAKO CorporationMWLSAB2
EGFRMM1:100Biogenex (San Ramon, CA)EPBALSAB+
ERMM1:50DAKO CorporationPCLSAB2
PRMM1:50DAKO CorporationPCLSAB2
pS2RP1:100Novocastra LaboratoriesNonePBALSAB2
Cathepsin DRP1:600DAKO CorporationPCLSAB2
E-cadherinMM1:3000Zymed Laboratories (San Francisco, CA)PCLSAB+
β-CateninGP1:2000Santa Cruz BiotechnologyPCPBALSAB+
  • a MM, mouse monoclonal; RP, rabbit polyclonal; GP, goat polyclonal; PC, pressure cooker; MW, microwave; E, enzyme digestion with pepsin; PBA, protein blocking agent; LSAB, labeled streptavidin-biotin.