Table 1

Characteristics of ovarian epithelial cells used for comparative gene expression studies

Pathological diagnosis of the primary cultures used for cDNA-RDA or cDNA filter array hybridization experiments are shown. Immunohistochemical analysis was performed to ensure that cells had homogeneous cytokeratin and vimentin staining with no factor VIII staining. p53 immunostaining results are also presented. Increasing intensities of staining are indicated from “+” to “+++”.

Culture typePathologic diagnosisCytokeratinVimentinFactor VIIIp53KaryotypesApplication
HOSE 231Normal++++NegaN/D46:XXcDNA-RDA
HOSE 250Normal++++NegN/D46:XXcDNA-RDA
CSOC 817Papillary serous++++Neg++45:X[12]/46XX[18] (60% of cells had one X)cDNA-RDA
CSOC 826Papillary serous++++Neg++47:XX,+mar[2]/46:XX[18] (10%+ marker)cDNA-RDA
HOSE 224Normal++++NegN/DN/DcDNA filter array hybridization
HOSE 246Normal++++NegNegN/DcDNA filter array hybridization
HOSE 253Normal++++NegN/DN/DcDNA filter array hybridization
HOSE 254Normal+++NegN/DN/DcDNA filter array hybridization
HOSE 256Normal++++NegN/DN/DcDNA filter array hybridization
CSOC 823Papillary serous++++NegNegN/DcDNA filter array hybridization
CSOC 824Papillary serous+++Neg+N/DcDNA filter array hybridization
CSOC 827Papillary serous++++NegNegN/DcDNA filter array hybridization
CSOC 834Papillary serous++++Neg+N/DcDNA filter array hybridization
CSOC 839Papillary serous+++NegNegN/DcDNA filter array hybridization
CSOC 843Papillary serous++++Neg++/+++N/DcDNA filter array hybridization
CSOC 846Papillary serous++++Neg++N/DcDNA filter array hybridization
CSOC 869Papillary serous+++++Neg++N/DcDNA filter array hybridization
CSOC 871Papillary serous+++++Neg++N/DcDNA filter array hybridization
CSOC 872Papillary serous+++Neg+N/DcDNA filter array hybridization
  • a Neg, negative; N/D, not determined.