Table 1

Oligonucleotide pairs used in reverse transcription PCR experiments

GenePCRPrimersGlycerol (%)Annealing Temperature (°C)Extension (sec)Cycles
NameSequence
BWR1C Primary395-Ex23F5′-cttaaggcgcccgtgcaa-3′12.5585035
Fe-24R5′-tgctacgcgagggcgagtt-3′
BWR1A Primary592-2F5′-gctggccgccacagaactta-3′12.5623035
592-2R5′-aggagcaggtagagcgcaa-3′
CDKN1C PrimaryHKIP2 1101F5′-agatcagcgcctgagaagtc-3′12.5593038
HKIP2 1308R5′-tgcggcagccgccgccggtt-3′
RET PrimaryRET 359F5′-gcatctctacggcacgtacc-3′7614035
RET 721R5′-agcaggcggaactggtggaa-3′
WT1 PrimaryWT1-262F5′-tgcctgagcgccttcactgt-3′7594035
WT1-713R5′-caggtcatgcattcaagctgg-3′
N-CAM PrimaryNCAM-Ig1F5′-ccaaaccagcagcggatctc-3′7594035
NCAM-Ig2R5′-ggacaggactatgaatcggac-3′
E-cadherin PrimaryECDH-Ex14F5′-ttcttcggaggagagcggtg-3′7615035
ECDH-Ex16R5′-ctccgtacatgtcagccagc-3′
PAX2 PrimaryPAX2-ex7F5′-gcacttgcgagctgacacct-3′7614022
PAX2-ex11R5′-cgttgtaggccgtgtactgg-3′
NestedPAX2-ex7F5′-gcacttgcgagctgacacct-3′12.5623035
PAX2-ex89R5′-gccatgtcacgaccagtcac-3′
GDNF PrimaryGDNF-10F5′-tgggatgtcgtggctgtctg-3′12.5615035
GDNF-547R5′-cgatgggtctgcaacatgcc-3′
NestedGDNF-10F5′-tgggatgtcgtggctgtctg-3′7615035
GDNF-Ex2R5′-ctgcatcgcaagagccgctg-3′