Table 1

In vivo interactions between hHR23A and p300 segments in the yeast two-hybrid system

Yeast strain Y187 was transformed with pGBKT7 bait construct and pGADT7 target construct. Colonies were streaked on synthetic dropout selection agar and assayed for β-galactosidase activity. Four colonies were assayed for each kind of transformant, and experiments were repeated three times.

Bait constructionTarget constructionβ-Galactosidase assaya
PGBKT7-hHR23A+
PGBKT7-hHR23A dc57±
pGADT7-p300 (1-140)
pGADT7-p300 (1-340)
pGADT7-p300 (1-595)
pGADT7-p300 (300-528)
pGBKT7-hHR23ApGADT7+
pGBKT7-hHR23ApGADT7-p300 (1-140)+
pGBKT7-hHR23A dc57pGADT7-p300 (1-140)±
pGBKT7-hHR23ApGADT7-p300 (1-340)+
pGBKT7-hHR23A dc57pGADT7-p300 (1-340)±
pGBKT7-hHR23ApGADT7-p300 (300-528)++
pGBKT7-hHR23ApGADT7-p300 (1-595)+++
pGBKT7-hHR23A dc57pGADT7-p300 (1-595)+
pGBKT7-LampGADT7-p300 (1-595)
  • a −, no evidence for color change of the yeast colony; ±, color of colony changed to very light blue at the end of 8-h test; +, ++, and +++, relative blue color intensities of the colonies.