Table 1

Frequencies of metaphases showing each type of plasmid localization in the mixture of stable transformants

PlasmidsOriMARSignals at DMsaFormation of HSRbGiant signals in interphasec
COLO 32ODM
 pEPBGOriPOriP6/45 (13%)0/45 (0%)NEd
 pSFVdhfr DHFR DHFR 28/47 (60%)5/47 (11%)NE
 pSFV-Vnonenone0/45 (0%)0/45 (0%)NE
 pNeo.Myc-2.4c-mycSV402/46 (4.3%)8/46 (17%)NE
 pNeo-VnoneSV400/31 (0%)0/31 (0%)0/562 (0%)
 pNeo.Myc ΔSVc-mycnone0/22 (0%)0/22 (0%)2/314 (0.4%)
 pNeo.Myc ΔSV AR1c-myc Igκ 8/25 (32%)6/25 (24%)82/553 (15%)
COLO 320HSR
 pSFVdhfr DHFR DHFR 8/40 (20%)0/26 (0%)
 pSFV-Vnonenone0/45 (0%)0/45 (0%)
 pNeo.Myc ΔSVc-mycnone0/32 (0%)3/512 (0.6%)
 pNeo.Myc ΔSV AR1c-myc Igκ 12/29 (41%)2/431 (0.5%)
  • a Frequencies of metaphases showing the plasmid localization at most of DMs among the metaphase.

  • b Frequencies of metaphases showing the formation of chromosomal HSR-like structure composed with plasmid sequences.

  • c Frequencies of interphase nuclei having the giant plasmid signal, which correspond to the HSR-like structure seen in the metaphase.

  • d NE, not examined.