Table 4

List of lac1 mutations observed in two mammary carcinoma cell lines, two primary carcinomas and primary culture of normal mammary epithelium

Codon no.Nt.a no.Sequence changeMutation typebAmino acid changeNo. of mutants
Mammary carcinoma cell linePrimarycarcinomaPrimary culture of normal mammary epithelium
PhIP12-1PhIP7-4
Wild typeMutationWild typeMutationEx.a 1Ex. 2Ex. 3Ex. 4Ex. 5Ex. 6Ex. 1Ex. 2Ex. 3Ex. 4Ex. 5Ex. 612-17-4
234GTG AAA CCAGTG AAC CCA1LysAsn5
541GTA ACG TTAGTA CCG TTA1ThrPro11
1056GTC GCA GAGGTC ACA GAG1AlaThr114
1057GTC GCA GAGGTC GAA GAG1AlaGlu1
1366TAT GCC GGTTAT GTC GGT1AlaVal11
1366TAT GCC GGTTAT GGC GGT1AlaGly4
1469GCC GGT GTCGCC GCT GTC1AlaGly1
1572GGT GTC TCTGGT GCC TCT1ValAla1
1674GTC TCT TATGTC CCT TAT1SerPro1
1675GTC TCT TATGTC TTT TAT1SerPhe1
1984CAG ACC GTTCAG AAC GTT1ThrIle112
1984CAG ACC GTTCAG ATC GTT1ThrAla1
1984,85CAG ACC GTTCAG ATA GTT4ThrIle111
2086ACC GTT TCCACC TTT TCC1ValPhe3
2089GTT TCC CGCGTT CCC CGC1SerPro1
2190GTT TCC CGCGTT TTC CGC1SerPhe11
2293TCC CGC GTGTCC CTC GTG1ProLeu2
25103GTG AAC CAGGTG AAG CAG1AsnLys11
27107CAG GCC AGCCAG CCC AGC1AlaPro1
35132ACG CGG GAAACG CGGAA2Frameshift (−1)3
36135CGG GAA AAACGG GAAAA2Frameshift (−1)11
36135CGG GAA AAACGG GAAAAAA3Frameshift (+1)12
38140AAA GTG GAAAAA CTG GAA1ValLeu11
38140AAA GTG GAAAAA ATG GAA1ValMet3
38141AAA GTG GAAAAA GGG GAA1ValGly1
47167AAT TAC ATTAAT GAC ATT1TyrAsp11
47167AAT TAC ATTAAT AAC ATT1ThrAsn2
47168AAT TAC ATTAAT TGC ATT1ThrCys3
49174ATT CCC AACATT CTC AAC1ProLeu7225
50176CCC AAC CGCCCC CAC CGC1AsnHis1
51179AAC CGC GTGAAC TGC GTG1ArgCys11
53185GTG GCA CAAGTG TCA CAA1AlaSer1
53188GTG GCA CAAGTG GTA CAA1AlaLeu11
56194CAA CTG GCGCAA GTG GCG1LeuVal11212
61210CAG TCG TTGCAG TAG TTG1SerAMB11
81270GTC GCG GCGGTC GTG GCG1AlaVal4
81270GTC GCG GCGGTC GAG GCG1AlaGlu11
87287CGC GCC GATCGC CCC GAT1AlaPro11
90296CAA CTG GGTCAA TTG GGT1LeuMet1
92302GGT GCC AGCGGTCCAGC2Frameshift (−1)1
92303GGT GCC AGCGGT GCAGC2Frameshift (−1)3
101329GAA CGA AGCGAA TGA AGC1ArgOPA121671
106345GAA GCC TGTGAA GCTGT2Frameshift (−1)1
114368AAT CTT CTCAATACTTCTC3Frameshift (+1)1
114369AAT CTT CTCAAT CGT CTC1LeuArg36211112241
118381CAA CGC GTCCAA CCC GTC1ArgPro1
123396CTG ATC ATTCTG ATTCATT3Frameshift (+1)1
148471TTT CTT GATTTT CGT GAT1LeuArg11
148471TTT CTT GATTTT CCT GAT1LeuPro1
160507ATT ATT TTCATT ATTTC2Frameshift (−1)1
167527GGT ACG CGAGGT GCG CGA1ThrGlu1
167528GGT ACG CGAGGT AAG CGA1ThrLys61
182572CAA ATC GTCCAA CTC GTC1IleLeu1
183575ATC GCG CTGATC CCG CTG1AlaPro1
187587GCG GGC CCAGCG CGC CCA1GlyArg1
201629GGC TGG CATGGCAT4Frameshift (−4)1
202633TGG CAT AAATGG CCT AAA1HisPro11
204640AAA TAT CTCAAA TAG CTC1TyrAMB222
218680GAA GGC GACGAA AGC GAC1GlySer1
224701TCC GGT TTTTCC CGT TTT1GlyArg21
225702TCC GGT TTTTCC GCT TTT1GlyAla1
227707TTT CAA CAATTT TAA CAA1GlnOCH111
231720ATC CAA ATGATC CGAAATG3Frameshift (+1)1
239743ATG CGC GCCATG CGGCC2Frameshift (−1)3
242753GCG ATG ATGGCG AAG ATG1MetLys1
253786GGC GCA ATGGGC GAA ATG1AlaGlu1
272843GTG GGA TACGTG GTA TAC1GlyLeu1
280867AGC TCA TGTAGC TAA TGT1SerOCH11
281869TCA TGT TATTCA CGT TAT1CysArg111
282874TGT TAT ATCTGT AAT ATC1ThrAsn1
285881CCG CCG TTACCG GCG TTA1ProAla7
296916CTG CTG GGGCTG CTGGGGG3Frameshift (+1)1
303936GAC CGC TTGGAC CCC TTG1ArgPro1
3421054GCG TTG GCCGCG TTGGGCC3Frameshift (+1)1
3761155ACC CCA GGCACC GAG GGC4ProGln2
No. of total mutants6867137946118568566
  • a Nt, nucleotide; Ex., experiment; M, no mutant.

  • b 1, one base substitution; 2, one base deletion; 3, insertion; 4, complex mutation.