Table 1

Tumor growth of HEK293 xenografts

Four cohorts of 15 mice each were inoculated with either 4 × 106 FAP-transfected HEK293 cells/mouse, 4 × 106 mock-transfected HEK293 cells/mouse, 1 × 107 FAP-transfected HEK293 cells/mouse, or 1 × 107 mock-transfected HEK293 cells/mouse. Mock transfection consisted of the negative control of HEK293 cells transfected with empty pcDNA3 vector. HEK293 cells transfected with FAP demonstrate a greater propensity for tumor development, a shortened latency period, and an enhanced growth rate compared with mock-transfected controls.

Cohort (15 mice/cohort)CellsNo. of inoculated cells/mouseNo. of mice developing tumorTumor volumea (mean ± SE)Days to palpable tumorsb (mean ± SD)
1HEK-FAP4 × 1068/15498.4 ± 182.423.2 ± 8.1
2HEK-Mock4 × 1062/15c49.1c ± 49.133 ± 2.8
3HEK-FAP1 × 10713/15898.6 ± 151.49 ± 5.9
4HEK-Mock1 × 1077/15c21.8c ± 9.034 ± 3.6
  • a Mean tumor volume of all mice measured on the day mice were culled. Mice were culled on day 42 for cohorts 1 and 2 and on day 38 for cohorts 3 and 4.

  • b Mean days from inoculation until tumor first palpable in mice developing tumors.

  • c P < 0.05 comparing cohorts 1 and 2 or cohorts 3 and 4.