Table 1

Time course of PSA expression in prostate cancer cells microinjected with monoclonal AR antibody

Laser scanning confocal microscopy was used to visualize PSA expression in microinjected cells. Prostate cancer cells were injected with AR antibody or heat-inactivated antibody. Cells were fixed 24, 48, 72, and 96 h after injection and stained to estimate PSA expression.

PSA expression
Time (h)24487296
LNCaP/AR antibody++++a++++−−−−
LNCaP/heat inactivated++++++++++++++++
LNCaP-RF/AR antibody+++++++−−−−
LNCaP-RF/heat inactivated++++++++++++++++
LNCaP-C4/AR antibody+++++++−−−−
LNCaP-C4/heat inactivated++++++++++++++++
  • a ++++, 100% PSA expression; +++, 75%; ++, 50%; +, 25%; −−−−, no PSA expression.