Table 3

Table of putative somatic HPRT in vivo hotspots

bp PositionaMutation typebSomatic mutations in vivocSequence contextdbp PositioneMutation typeSomatic mutations in vivoSequence context
41del 92GAA CCA GGT TAT GAC404a > t2aag GAT ATA
47g > a3CCA GGT TAT418g > c2ACT GGC AAA
65t > c3TTA TTT TGC419g > a2ACT GGC AAA
74c > g3ATA CCT AAT463c > t3AAT CCA AAG
74c > t3ATA CCT AAT464c > t3AAT CCA AAG
85g > a2TAT GCT GAG464c > g2AAT CCA AAG
135g > c2tag G ACT481g > c3GTC GCA AG g
135g > t2tag G ACT484a > c2GCA AG gta
143g > a13GAA CGT CTTIVS6+ 1g > a3A AG gtat
143g > t2GAA CGT CTT486c > g4cag C TTG
145c > t2CGT CTT GCT496a > g2GTG AAA AGG
146t > g6CGT CTT GCT508c > t15CCA CGA AGT
146t > c2CGT CTT GCT527c > g3AAG CCA GAC
151c > t5GCT CGA GAT529g > t2CCA GAC T gta
194t > c2GCC CTC TGT530a > t2CCA GAC T gta
197g > a29CTC TGT GTGIVS7+ 1g > t2GAC T gtaa
197g > t6CTC TGT GTG538g > a2GTT GGA TTT
207f−7AA(G GGG GG)C TAT539g > a4GTT GGA TTT
207f+ 12AA(G GGG GG)C TAT542t > a2GGA TTT GAA
208g > a6AAG GGG GGC543t > g3GGA TTT GAA
209g > a5AAG GGG GGC551c > t5ATT CCA GAC
211g > c3AAG GGG GGC551c > g2ATT CCA GAC
212g > a6GGG GGC TAT554a > t2CCA GAC AAG
213f− 12GGGCTA563t > a2TTT GTT GTA
222c > a3AAA TTC TTT568g > c2GTA GGA TAT
222c > g4AAA TTC TTT568g > a7GTA GGA TAT
233t > c2GAC CTG CTG573t > a3GGA TAT GCC
295t > g2GAT TTT ATC574g > c2TAT GCC CTT
325c > t2GAC CAG TCA580g > a3CTT GAC TAT
368c > g3CTC TCA ACT586a > c2TAT AAT GAA
IVS4+ 1g > a2AAG gtat592t > g2GAA TAC TTC
IVS4+ 2t > a2AAG gtatg599g > a3TTC AGG GAT
389t > a2AAT GTC TTG601g > t2AGG GAT TTG
400g > a4GTG GAA gta602a > t2AGG GAT TTG
IVS5+ 1g > a2GAA gtaa606g > c3GAT TTG AAT
IVS5− 1g > a2aaag GATIVS8+ 1g > a5AAT gtaa
403g > a2aag GAT ATAIVS8+ 1g > a5AAT gtaa
404a > g3aag GAT ATA
  • a Column 1, hot spot position (A of ATG is nucleotide 1 for mutations occurring in coding sequences; intron mutations are represented by intron number and donor or acceptor site).

  • b Column 2, mutation type on the untranscribed DNA strand.

  • c Column 3, number of times the hot spot mutation was observed out of 458 6TGR mutants from peripheral T lymphocytes of normal individuals.

  • d Column 4, sequence position. Bold characters indicate the changed bp or sequence.

  • e Columns 5–8, Columns 1–4 repeated.