Table 2

Parameters of the TPZ transport model for HT29 MCLs

The model is described in “Appendix II.” The other parameters of the transport model are the TPZ metabolism parameters, determined in single cell suspensions (Table 1) <$REFLINK> and scaled to the cell density in MCLs using φc.

ParameterUnitsDescriptionValue ± SE (n)
Ds Urea (Mr 60)cm2s−1 × 106Diffusion coefficient for urea in Teflon support2.08 ± 0.05 (19)
Ds TPZ (Mr 178)cm2s−1 × 106Diffusion coefficient of TPZ in Teflon support1.26 ± 0.04 (5)
DMCL Ureacm2s−1 × 106Diffusion coefficient of urea in HT29 MCL0.450 ± 0.023 (6)
DMCL TPZcm2s−1 × 106Diffusion coefficient of TPZ in HT29 MCL0.40 ± 0.01 (12)
φcIntracellular volume fraction (calculated)0.517 ± 0.017 (20)
φeIntracellular volume fraction (experimental)0.508 ± 0.040 (6)