Table 2

Multiple cytokine secretion in serum from mice that received injections of mIL-21

C57BL/6 mice received i.v. injections of either saline alone, 20 μg of pORF, or mIL-21 plasmid DNA, respectively, on day 0. Mouse serum was collected 16 h and 4 and 8 days after DNA administration and subjected to multiple cytokine immunoassays (expressed as pg/ml serum). Three mice were in each group at each point. Positive controls were from serum samples collected from mice that received injections of 1 μg of mIL-2, mIL-4, mIL-10, and mIL-12 plasmid DNA, respectively. Data represent one of two experiments with similar results.

TreatmentDayIL-1βIL-2IL-4IL-5IL-6IL-10IL-12IFN-γTNFα
Saline16 h108 ± 24258 ± 720 ± 468 ± 35160 ± 2934 ± 1038 ± 633 ± 750 ± 21
pORF16 h137 ± 57288 ± 6224 ± 986 ± 45308 ± 204335 ± 46948 ± 2151 ± 2475 ± 37
mIL-2116 h155 ± 60297 ± 3428 ± 6111 ± 37296 ± 54248 ± 3246 ± 960 ± 8112 ± 51
None16 h89 ± 31275 ± 1118 ± 143 ± 19206 ± 15849 ± 4043 ± 850 ± 36307 ± 488
Saline4161 ± 83393 ± 10239 ± 9151 ± 61279 ± 14985 ± 3655 ± 2070 ± 16139 ± 44
pORF4161 ± 36292 ± 7325 ± 766 ± 17124 ± 2747 ± 1853 ± 849 ± 878 ± 45
mIL-214185 ± 138306 ± 6631 ± 1182 ± 44154 ± 76276 ± 14658 ± 3250 ± 2681 ± 31
Saline8232 ± 281205 ± 17022 ± 1595 ± 47146 ± 8644 ± 2436 ± 2540 ± 3083 ± 36
pORF8137 ± 20276 ± 2728 ± 490 ± 19130 ± 1550 ± 944 ± 2448 ± 1178 ± 22
mIL-218154 ± 111236 ± 17724 ± 1595 ± 15834 ± 1269413 ± 48952 ± 37253 ± 37598 ± 30
mIL-216 h2163693550.8532.81965116.725101.275
mIL-416 h6961142716118205502371142
mIL-1016 h4917640815224733.81493527270681
mIL-1216 h56308.8822351843990222.167