Table 1

Analysis of TRAIL resistance in human NB cells

Cell LineTRAIL sensitivityaCaspase8bSurface TR2cSurface TR1cC-FLIPd
A4573e++++/
SKNAS++++
CHP212++++
KCNR++++
BE2++
NBLS++
LAN5+
SKNDZ+
SKNLE+++
SY5Y+
CHP382+
CHP404+
IMR32+
LAN1+
CHP126
CHP134B
NGP
  • a + indicates a decrease in cell viability ≥20% in TRAIL treatment compared to control as assessed by MTT assay.

  • b As detected by Western analysis after IFN-γ treatment.

  • c + represents a minimum shift of 5 units in mean channel fluorescence intensity above isotype control staining as evaluated by FACS analysis.

  • d As detected by RT-PCR.

  • e A known TRAIL sensitive Ewing’s sarcoma cell line as positive control.