Table 1

Inhibition of VacA-dependent apoptosis by VacA Δ6-27a

SamplebFold increase in DNA fragmentation relative to:
AGS alonecAGS + bufferc
Wild-type VacA (5 μg/ml)6.5 ± 0.4d6.5 ± 0.2d
VacA Δ6-27 (5 μg/ml)0.8 ± 0.10.6 ± 0.06
Wild-type VacA (5 μg/ml)+ VacA Δ6-27 (5 μg/ml)0.9 ± 0.011.4 ± 0.3
  • a Apoptosis was determined by DNA fragmentation ELISA after 48 h of coculture in the presence of ammonium chloride (5 mm).

  • b Wild-type VacA and VacA Δ6-27 were acid-activated before incubation with AGS cells.

  • c Mean ± SD of two independent experiments.

  • d P < 0.05 versus controls.