Table 3

In vitro uptake and retention of boronated dThd analogues

CompoundL929 Wild-typeL929 TK
Uptake (μmol/L)Retention (%)Uptake (μmol/L)Retention (%)
4 69.4 ± 11.04512.9 ± 7.50
6 92.0 ± 20.0299.3 ± 2.70
10 91.1 ± 12.0466.0 ± 2.40
12 160.1 ± 17.0294.8 ± 1.50
  • NOTE. Cells were incubated in the presence of 17.5 μmol/L of tested compounds for 24 hours at 37°C. Subsequently, the cells were harvested, and boron content was determined by means of DCP-AES and expressed as micrograms of B/109 cells. Retention was determined by propagating the cells in compound-free medium for additional 12 hours. Data represent the mean ± SDs of three independent experiments.