Table 1

Effect of P15-Tat on the cell viability in different tumor cell lines

Tumor cell lineOriginIC50 (μmol/L)*
TC-1Mouse lung HPV16-transformed cells 71 ± 15
H-125Human non–small-cell lung cancer36 ± 9
H-82Human small-cell lung cancer20 ± 3
SiHaHuman cervical carcinoma135 ± 10
CaSkiHuman cervical carcinoma182 ± 6
HeLaHuman cervical carcinoma136 ± 16
JurkatHuman T-cell leukemia89 ± 3
  • NOTE. P15-Tat was added to 10,000 cells in a range of concentrations from 0 to 200 μmol/L. After 72 hours of incubation, cell viability was determined by adding 20 μl of MTS solution. Finally, absorbance at 492 nm was taken, and the IC50 values were calculated from the growth curves.

  • * Mean ± SD of three determinations. Reproducible values were observed from two different experiments.

  • Obtained and provided by Dr. T. C. Wu