Table 1.

Neutral sugar compositions of gp96

Source of gp96Sugar residue content/μg protein
Fuc (nmol)Gal (nmol)Glc (nmol)Man (nmol)
Rat
    Normal rat prostate5.00 ± 0.5005.38 ± 0.0885.00 ± 0.03817.50 ± 0.375
    Dunning G2.50 ± 0.2502.50 ± 0.0873.75 ± 0.1308.75 ± 0.275
    MAT-LyLu0.30 ± 0.0871.63 ± 0.2501.63 ± 0.38015.00 ± 0.250
Human
    LnCaP1.63 ± 0.0873.50 ± 0.0875.00 ± 0.08615.00 ± 0.250
    DU145ND (∼0)2.75 ± 0.0861.63 ± 0.08017.50 ± 0.300
  • NOTE: All values are in nmol ± SD. Quantitation of all neutral monosaccharide contents was done by HPAE-PAD analyses of the TFA hydrolysates derived from rat ( Fig. 3A) and human ( Fig. 4A) gp96 samples. For each compositional data, the mean of the three values obtained by triplicate analysis has been reported along with the corresponding SD. All associated probability values were checked by ANOVA to ensure that all monosaccharide composition data are within statistically significant range of variation (P < 0.05). As stated in Materials and Methods, due to batch-to-batch variation of the reference, the compositional data reported here may not be absolute. However, the contents are reliable relative to each other because the same batch of the reference was used throughout the study.

    Abbreviation: ND, not detectable.