Table 2.

Amino sugar compositions of the gp96

Source of gp96Sugar residue content/μg protein
GalNAc (nmol)GlcNAc (nmol)
Rat
    Normal rat prostate3.50 ± 0.12513.75 ± 0.250
    Dunning G2.00 ± 0.08815.00 ± 0.500
    MAT-LyLu1.38 ± 0.0631.63 ± 0.087
Human
    LnCaP1.50 ± 0.08725.00 ± 0.875
    DU1450.18 ± 0.0120.63 ± 0.037
  • NOTE: All values are in nmol ± SD. Quantitation of all amino monosaccharide contents was done by HPAE-PAD analyses of the HCl hydrolysates, derived from rat ( Fig. 3B) and human ( Fig. 4B) gp96 samples. For each compositional data, the mean of the three values obtained by triplicate analysis has been reported along with the corresponding SD. All associated probability values were checked by ANOVA to ensure that all monosaccharide composition data are within statistically significant range of variation (P < 0.05).