Table 2.

Effects of osteonectin expression on MDA-231 metastasis of cells infected with either Add1312 (ctrl) or AdCMV-OSN (OSN)

OrganCtrl (0) OSN (0.3) OSN (1.5) OSN (11.5)
Pancreas4.0 (1.8)2.7 (1.3)0.4 (0.2)1.5 (1.0)
Liver2.7 (0.9)2.4 (0.9)1.1 (0.4)0.4 (0.2)
Lung12.7 (3.0)15.5 (4.4)9.6 (2.9)4.6 (3.3)
Heart2.2 (0.6)2.3 (0.5)1.9 (0.7)0.5 (0.1)
Kidney3.5 (0.9)11.1 * (3.1)5.6 (2.1)1.2 (0.5)
Ribs3.1 (1.0)0.2 * (0.1)0.2 * (0.1)1.0 (0.5)
Spine1.9 (0.6)0.5 (0.2)0.6 (0.2)1.0 (0.4)
Mandible0.6 (0.1)0.2 (0.1)0.7 (0.2)0.2 (0.1)
Skull1.8 (0.6)1.8 (0.6)1.7 (0.4)0.4 (0.2)
Hind limbs1.5 (0.3)1.0 (0.4)1.7 (0.5)0.5 (0.2)
Fore limbs0.7 (0.3)0.4 (0.2)0.9 (0.2)0.2 (0.1)
Overall metastases34.8 (6.2)40.6 (6.5)28.7 (4.1)10.8 * (3.9)
Bone metastases9.6 (2.3)4.0 (1.2)5.9 (1.2)3.4 * (1.1)
  • NOTE: Values shown are mean tumor number/mouse ± SE.

    Ctrl, n = 27; OSN (0.3), n = 20; OSN (1.5), n = 19; OSN (11.5), n = 27.

  • * P < 0.05 compared with control using one-way ANOVA with Bonferroin's multiple comparison post-test.

  • Micrograms of osteonectin secreted/106 cells/24 h.