Table 1.

Biodistribution of 125I and 131I-labled T84.66 scFv-Fc fragments in BALB/c mice

Organ (%ID/g)024612244872
125I-scFv-Fc wild-type
Blood45.7 (2.86)39.2 (1.17)34.4 (1.21)30.3 (0.61)25.6 (0.70)21.4 (0.76)19.9 (1.17)19.0 (0.54)
Liver10.7 (0.78)9.16 (0.24)8.31 (0.14)7.28 (0.36)5.43 (0.18)5.52 (0.35)4.72 (0.15)4.57 (0.27)
Spleen16.3 (1.42)13.3 (0.39)10.9 (0.34)9.90 (1.00)6.98 (0.35)6.49 (0.27)5.89 (0.46)6.18 (0.35)
Kidney10.7 (0.95)9.65 (0.90)8.89 (0.76)7.82 (0.49)7.58 (0.23)5.89 (0.25)5.99 (0.32)5.86 (0.23)
Lung15.3 (1.58)13.8 (0.52)13.2 (0.44)13.0 (0.61)10.8 (0.66)8.84 (0.36)9.22 (0.30)9.49 (0.35)
Bone4.19 (0.39)4.84 (0.33)3.90 (0.14)3.90 (0.15)3.29 (0.18)2.93 (0.09)2.69 (0.27)2.64 (0.13)
Carcass1.94 (0.13)2.44 (0.07)2.52 (0.06)2.50 (0.04)2.62 (0.04)2.63 (0.10)2.41 (0.06)2.68 (0.06)
125I-scFv-Fc H435R
Blood46.3 (1.38)35.1 (2.41)29.9 (0.73)28.1 (0.67)24.7 (0.98)21.1 (0.49)17.1 (0.42)15.4 (0.24)
Liver12.8 (0.29)10.3 (0.56)9.27 (0.36)8.80 (0.22)6.06 (0.34)5.43 (0.35)4.22 (0.12)3.86 (0.20)
Spleen16.8 (0.66)13.3 (1.06)11.5 (0.62)10.1 (0.56)8.01 (0.37)7.05 (0.46)5.45 (0.34)4.86 (0.24)
Kidney12.3 (0.52)10.8 (0.67)9.49 (0.40)8.66 (0.59)7.17 (0.50)5.76 (0.30)4.51 (0.23)3.90 (0.24)
Lung14.1 (0.80)13.6 (1.24)11.5 (0.59)11.4 (0.43)10.6 (0.66)8.74 (0.27)7.43 (0.17)6.58 (0.24)
Bone4.39 (0.45)4.34 (0.32)3.88 (0.31)3.21 (0.17)3.35 (0.19)2.87 (0.12)2.25 (0.05)2.11 (0.14)
Carcass1.69 (0.12)2.32 (0.08)2.37 (0.08)2.39 (0.06)2.55 (0.07)2.42 (0.06)2.23 (0.05)2.03 (0.07)
131I-scFv-Fc H435Q
Blood48.9 (1.20)35.6 (2.49)30.0 (0.81)27.4 (0.66)24.5 (0.98)19.7 (0.43)14.3 (0.43)11.5 (0.21)
Liver9.46 (0.47)7.41 (0.43)6.85 (0.20)6.56 (0.23)5.06 (0.29)4.05 (0.22)2.90 (0.12)2.34 (0.12)
Spleen12.2 (0.44)9.06 (0.82)8.68 (0.39)7.67 (0.51)6.40 (0.33)5.64 (0.40)3.98 (0.30)3.17 (0.17)
Kidney12.0 (0.44)9.56 (0.64)8.70 (0.43)7.77 (0.57)6.69 (0.49)5.07 (0.30)3.53 (0.19)2.74 (0.18)
Lung14.0 (0.81)12.7 (1.20)10.8 (0.53)10.3 (0.45)9.89 (0.64)7.84 (0.28)5.65 (0.15)4.55 (0.19)
Bone4.08 (0.37)3.87 (0.29)3.51 (0.24)2.85 (0.15)3.17 (0.19)2.60 (0.13)1.81 (0.05)1.51 (0.09)
Carcass1.84 (0.12)2.44 (0.07)2.50 (0.09)2.49 (0.07)2.59 (0.08)2.40 (0.07)1.94 (0.05)1.61 (0.06)
131I-scFv-Fc I253A
Blood48.1 (3.17)33.1 (0.97)27.1 (1.06)2.5 (0.54)17.4 (0.46)12.7 (0.44)9.58 (0.63)7.76 (0.22)
Liver11.1 (0.73)7.47 (0.14)6.17 (0.17)5.15 (0.26)3.55 (0.09)3.11 (0.16)2.14 (0.10)1.81 (0.10)
Spleen13.9 (1.17)9.64 (0.22)7.78 (0.31)6.60 (0.60)4.25 (0.20)3.58 (0.15)2.58 (0.19)2.35 (0.13)
Kidney11.2 (0.91)7.98 (0.65)6.79 (0.59)5.70 (0.37)4.96 (0.18)3.46 (0.14)2.79 (0.18)2.39 (0.10)
Lung14.7 (1.53)11.5 (0.39)10.2 (0.39)9.38 (0.44)7.03 (0.43)5.16 (0.21)4.11 (0.16)3.64 (0.15)
Bone3.92 (0.37)3.95 (0.25)3.05 (0.15)2.85 (0.09)2.17 (0.13)1.72 (0.05)1.27 (0.14)1.14 (0.07)
Carcass2.02 (0.17)2.53 (0.07)2.41 (0.10)2.17 (0.04)2.01 (0.03)1.87 (0.08)1.35 (0.05)1.39 (0.04)
125I-scFv-Fc H310A
Blood53.2 (2.08)31.1 (0.57)5.0 (0.74)21.6 (0.55)14.4 (0.52)9.85 (0.32)6.18 (0.19)4.15 (0.26)
Liver16.8 (0.58)7.90 (0.11)6.88 (0.22)6.23 (0.41)3.61 (0.07)2.52 (0.10)1.52 (0.05)1.15 (0.06)
Spleen13.5 (0.65)7.34 (0.16)7.18 (0.19)5.82 (0.50)3.75 (0.10)2.84 (0.18)1.71 (0.11)1.26 (0.09)
Kidney16.2 (0.52)8.63 (0.29)7.10 (0.41)6.53 (0.29)4.23 (0.08)2.86 (0.04)1.87 (0.07)1.32 (0.06)
Lung14.6 (0.79)11.8 (0.50)9.22 (0.28)8.40 (0.20)6.05 (0.29)4.13 (0.15)2.57 (0.06)1.82 (0.13)
Bone4.48 (0.18)3.52 (0.19)3.08 (0.22)2.59 (0.16)2.00 (0.10)1.33 (0.06)0.76 (0.03)0.64 (0.03)
Carcass2.50 (0.15)2.70 (0.12)2.73 (0.12)2.57 (0.17)2.02 (0.03)1.66 (0.05)1.21 (0.05)0.95 (0.03)
131I-scFv-Fc H310A/H435Q
Blood57.4 (2.00)32.0 (0.64)24.0 (0.75)19.4 (0.50)0.7 (0.46)4.47 (0.18)1.06 (0.03)0.32 (0.03)
Liver13.5 (0.48)6.85 (0.07)5.41 (0.18)4.46 (0.33)2.18 (0.05)0.97 (0.04)0.33 (0.01)0.20 (0.01)
Spleen12.6 (0.63)6.88 (0.18)6.39 (0.11)4.95 (0.43)2.63 (0.07)1.25 (0.10)0.31 (0.02)0.10 (0.01)
Kidney12.6 (0.58)7.85 (0.29)6.18 (0.35)5.41 (0.27)2.94 (0.06)1.25 (0.04)0.35 (0.01)0.14 (0.01)
Lung14.2 (0.76)11.5 (0.53)8.67 (0.30)5.76 (1.46)4.56 (0.34)1.89 (0.07)0.48 (0.01)0.18 (0.02)
Bone4.28 (0.14)3.51 (0.18)3.02 (0.23)2.43 (0.22)1.59 (0.09)0.67 (0.05)0.19 (0.02)0.03 (0.01)
Carcass2.27 (0.16)2.57 (0.12)2.59 (0.13)2.33 (0.16)1.60 (0.05)0.94 (0.04)0.51 (0.05)0.35 (0.02)
  • NOTE: Groups of five mice were analyzed at each time point. Organ uptake is expressed as %ID/g [mean (SE)].