Table 2.

Estimated values of blood half-times for the T84.66 cFv-Fcγ1 fragments

Antibody fragmentt1/2α (h)Aα * (%ID/g)t1/2β (h)Aβ (%ID/g)Area under the curve Mean residence time (h)
Wild-type4.2223.328922.49,480411
H435R1.8719.583.426.63,250118
H435Q1.5320.952.628.02,17074
I253A2.3128.951.718.81,49770
H310A1.6632.627.220.287336
H310A/H435Q0.8625.28.0032.340211
  • * Amplitudes of the two components are given by Aα and Aβ, where the sum of Aα and Aβ is the total %ID/g.

  • Area under the curve is a time integral of the blood uptake (%ID/g × h).

  • Mean residence time used to give a single variable for blood clearance.