Table 2.

OTX2 expression in medulloblastomas

Sample IDDiagnosis *Fold of OTX2 expression by Q-PCR OTX2 SAGE tag numbers per 200,000 tags
Normal cerebellum1.00NA
Medulloblastoma cell lines
    D487NA128.00NA
    D721NA90.62NA
    D425NA72.17NA
    D283NA56.7654
    D384Anaplastic, large cell42.83NA
    D556Anaplastic, large cell35.59NA
    D458NA35.03NA
    D341NA13.1072
    D581NA0.91NA
    D324NA0.10NA
    MHH1NA0.010
    MCD-1NA0.01NA
    UW228NA0.00NA
Medulloblastoma primary tumors
    TB1244Anaplastic, large cellNA77
    TB285AnaplasticNA45
    TB1273AnaplasticNA31
    TB476Anaplastic, large cell328.43NA
    TB2235Classic, with focal anaplasia124.68NA
    TB2227Anaplastic100.50NA
    TB2223Desmoplastic, nodular85.36NA
    TB2226Anaplastic57.57NA
    TB1377Anaplastic, large cell54.05NA
    TB1961Anaplastic, large cell53.68NA
    TB830Classic44.09NA
    TB54Classic, with focal anaplasia37.9322
    TB1339Anaplastic, large cell36.499
    TB2178Desmoplastic, with focal anaplasia18.10NA
    TB771Anaplastic, large cell3.190
    TB2224Desmoplastic, nodular1.52NA
    TB2222Desmoplastic, nodular0.91NA
    TB1341Desmoplastic, nodular0.31NA
    TB2025Desmoplastic0.18NA
    TB876Desmoplastic, nodular0.170
TB100Classic0.050
    TB2228Classic0.02NA
    TB1423Desmoplastic0.00NA
  • Abbreviation: NA, data not available.

  • * Histopathologic categories were defined by two board-certified neuropathologist (REM, TJP) at Duke as follows. Desmoplasia: connective tissue and/or pericellular reticulin fibers distributed throughout the tumor. Large cell: cells with large open nuclei with or without enlarged nucleoli. Anaplasia: high mitotic activity, nuclear crowding, extensive apoptosis and necrosis, or extensive large cell change. Classic pattern: low to moderate mitotic activity, apoptotic activity, and necrotic foci.

  • Fold of OTX2 expression was defined as the ratio of OTX2 expression in tumor relative to normal.

  • The SAGE data were obtained from the National Center for Biotechnology Information Cancer Genome Anatomy Project repository and the OTX2 SAGE tag, and its presence in the tumors was identified by using the SAGE Anatomic Viewer.