Table 1.

Oligonucleotides used as primers in PCR and as probes in filter hybridization

SV40 DNA regionsOligonucleotides *Reference positionSize (bp)
Tag NH2PYV.for: 5′-TAGGTGCCAACCTATGGAACAGA-3′nt 4,574-4,55262172
PYV.rev: 5′-GAAAGTCTTTAGGGTCTTCTACC-3′nt 4,403-4,425
SV probe: 5′-ATGTTGAGAGTCAGCAGTAGCC-3′nt 4452-4473
RegulatoryRA3: 5′-GCGTGACAGCCGGCGCAGCACC-3′nt 358-33664483
RA4: 5′-GTCCATTAGCTGCAAAGATTCCTC-3′nt 5,118-5,142
R probe: 5′-AATGTGTGTCAGTTAGGGTGTG-3′nt 264-244
Human p53 gene exons 7-8For: 5′-ATCCTGAGTAGTGGTAATCT-3′nt 14,441-14,46055151
Rev: 5′-TACCTCGCTTAGTGCTCCCT-3′nt 14,591-14,572
  • * Oligonucleotides used as primers in PCR and as probes in filter hybridizations.

  • Reference nucleotide positions in SV40 strain 776 and human p53 gene as a control of DNA suitability (European Molecular Biology Laboratory/Genbank accession no. X54156).

  • PCR annealing temperature (°C).