Table 1.

Analyses for cell proliferation in mammary glands of pubertal wild-type and Tg (c-neu)

GenotypeStructuresEndocrine statusBrdUrd-positive cells (% total cells)
IIIIII
Wild typeNormal TEBIntactNF5.4 ± 2.3123.9 ± 3.03
OvxNFNFNF
Normal ductsIntact0.2 ± 0.152.8 ± 0.59NF
Ovx0.1 ± 0.061.7 ± 0.31NF
c-neuNormal TEBIntactNFNFNF
Abnormal TEBIntactNF7.3±1.19 *21.3 ± 2.32
OvxNF1.4 ± 0.35NF
Normal DuctsIntact0.3 ± 0.162.7 ± 0.47NF
Ovx0.04 ± 0.032.2 ± 0.46NF
Hyperplastic DuctsIntact0.2 ± 0.103.5 ± 0.4230 ± 4.02
Ovx0.1 ± 0.044.1 ± 0.61NF
  • NOTE: Mammary glands from intact and ovariectomized wild-type mice and Tg (c-neu) were analyzed for BrdUrd immunoreactivity as described in text. Based on the number of BrdUrd-positive cells, each structure was roughly classified into three groups: BrdUrd labeling index <1 (group I), 1 to 10 (group II), and >10 (group III).

    Abbreviation: NF, not found.

  • * Abnormal TEB of ovariectomized Tg (c-neu) is significantly lower compared with its counterpart in intact mice (P < 0.05).