Table 1.

Checkerboard analysis of supernatant of AdSDF-1α-infected A549 cells

Lower chamber (%)Upper chamber (%)
02550100
04 ± 14 ± 15 ± 24 ± 2
25321 ± 3673 ± 926 ± 410 ± 3
50266 ± 27178 ± 16103 ± 1017 ± 4
100242 ± 30131 ± 11133 ± 1353 ± 8
  • NOTE: Different dilutions of supernatants of AdSDF-1α-infected A549 cells were prepared and placed in upper and lower chambers. Dendritic cells were placed in the upper chamber. The chamber was incubated for 90 minutes at 37°C. Migration activity was expressed as the number of cells in high-power fields, which migrated into the lower chamber.