Table 4.

Quantification of immunohistochemical staining

Absorbance* (mean ± SD)
p-EGFREGFRTGF-αp-Aktp-MAPK
L3.6p1
 Control1.7 ± 0.51.5 ± 0.22.3 ± 0.91.7 ± 0.33 ± 0.6
 ZD18390.5 ± 0.2†1.5 ± 0.71.2 ± 0.4†0.7 ± 0.1†0.3 ± 3.4†
MiaPaCa-2
 Control1.2 ± 0.50.8 ± 0.20.6 ± 0.11.5 ± 0.51.5 ± 0.5
 ZD18391.3 ± 0.50.7 ± 0.50.7 ± 0.11.8 ± 0.51.5 ± 0.4

*The absorbance of six positive cells per field in 10 random 0.159 mm2 fields at × 100 magnification of different tumor tissues were measured using Image Quant analyzer and Optima Image software.

P < 0.001 compared with the control group.