Treatment | Doubling time (h) | ||||
---|---|---|---|---|---|
Colo205 (B-RAF^{V600E}) | H1666 (B-RAF^{G466V}) | A549 (K-RAS^{G12S}) | H1703 * | H157 * (K-RAS^{G12R} and PTEN^{G251C}) | |
Control | 27 | 62 | 21 | 57 | 34 |
CI-1040 | 173 | 120 | 32 | 84 | 125 |
AP23573 | 56 | 81 | 32 | 141 | 51 |
Combination | 332 | 385 | 47 | 288 | 357 |
NOTE: Cells were seeded into 24-well plates and treated with CI-1040 and AP23573 alone, and in combination, at doses that resulted in synergism (see Fig. 2). After approximately three cell doublings, the number of adherent viable cells was determined using a coulter counter. The doubling time (h) was calculated according to the formula, t*ln(2)/ln(A/Ao), where A is the cell number at time t; Ao is the initial cell number. RAS, RAF, and PTEN genotypes are indicated.
↵* CA-AKT.