Table 2.

Peptides identified for 14-3-3𝛉 isoforms

Two-dimensional SpotUnique peptidesPeptide probabilityΔMassSequencePeptide positionTotal MS events
Spot 190.32400.001LAEQAER12–181
0.99940.000AVTEQGAELSNEER28–413
0.9303−0.001NLLSVAYK42–491
0.99960.000YLIANATNPESK104-1152
0.54730.002YLAEVACGDDR128–1381
0.99970.003QTIDNSQGAYQEAFDISK140–15710
0.56950.001EMQPTHPIR159–1672
0.99990.002TAFDEAIAELDTLNEDSYK194–2126
0.99740.000DSTLIMQLLR213–2222
Spot 460.99970.003YLIANATNPESK104–1152
0.55740.002MKGDYFR121–1272
0.99980.007QTIDNSQGAYQEAFDISK140–1577
0.58960.003EMQPTHPIR159–1674
0.99990.003TAFDEAIAELDTLNEDSYK194–2124
0.99300.003DSTLIMQLLR213–2224
  • NOTE: Spot 1 and spot 4 were recognized by the 14-3-3 monoclonal antibody and lung cancer sera in A549 two-dimensional blots ( Fig. 1D). Peptides at the NH2-terminal portion identified only in spot 1 are in boldface. Three MS runs from three independent spot digestions were performed. ProteinProphet Score was 1.0 representing an error rate <1%.