Table 1.

Effect of Flt-1 signals in macrophages on Gl261 intracranial gliomas

Analysis parametersIntracranial implanted tumor in bone marrow chimeras
Gl261-WTGL261-VEGFGL261-PlGF
Flt-1 TK+/+Flt-1 TK−/−Flt-1 TK+/+Flt-1 TK−/−Flt-1 TK+/+Flt-1 TK−/−
Tumor volume (mm3) [MRI]
    Day 1215.9 ± 9.9 *2.76 ± 1.042.1 ± 2725.1 ± 18.426.6 ± 12.3 *10 ± 7.9
(n = 5)(n = 5)(n = 5)(n = 6)(n = 6)(n = 8)
    Day 2067.7 ± 37.07 *21.25 ± 13
(n = 5)(n = 5)
Tumor volume (mm3) [histology]
    Day 1298.1 ± 40.792 ± 77.420.9 ± 17.18.1 ± 7.9
(n = 5)(n = 5)(n = 5)(n = 6)
    Day 2082.6 ± 51.7 15.4 ± 17.4
(n = 10)(n = 10)
Vessel density (%)9.21 ± 1.92 *5.28 ± 1.1811.1 ± 1.4110.62 ± 1.69.31 ± 2.297.69 ± 1.1
(n = 4)(n = 4)(n = 6)(n = 4)(n = 3)(n = 5)
GFP infiltration (%)1.27 ± 0.860.69 ± 0.253.9 ± 0.3 1.11 ± 0.483.43 ± 0.73 *2.33 ± 0.43
(n = 4)(n = 4)(n = 4)(n = 4)(n = 5)(n = 6)
Vessel permeability (kep [1 min−1])3.52 ± 3.43.35 ± 1.66.7 ± 4.96.3 ± 4.82.71 ± 2.12.7 ± 2.36
(n = 3)(n = 4)(n = 4)(n = 4)(n = 2)(n = 2)
WBC count (Ts/μL)5.04 ± 1.283.38 ± 0.464.40 ± 2.16.81 ± 1.365.4 ± 3.23.93 ± 0.99
(n = 5)(n = 5)(n = 5)(n = 4)(n = 4)(n = 4)
  • NOTE: Data represent mean ± SD. Tumor volume was assessed by means of MRI and histologic analysis. Vessel density was determined in at least eight CD31-stained sections per animal. GFP infiltration was evaluated in at least eight tumor-containing coronal sections per animal. Vessel permeability was determined in vivo by DCE-MRI.

  • * P < 0.05.

  • P < 0.01.