Table 1.

Effect of increased AHA1 protein expression on the sensitivity of human colon cancer cells to the HSP90 inhibitor 17-AAG

Overexpressing clonesEmpty vector clones
Parental HT29F1007-2F1007-3F1005-EF1005-H
17-AAG (nmol/L)5.9 (±0.81)3.2 (±0.68)5.3 (±0.58)5.1 (±1.93)6.5 (±0.81)
Parental HCT116F1007-CF1007-GF1005-2F1005-3
17-AAG (nmol/L)22 (±3.6)17 (±3.1)16 (±3.4)16 (±1.4)17 (±3.1)
  • NOTE: Ninety-six hour SRB growth inhibition assays were performed with parental HT29 cells, HCT116 cells, or cells transfected with either the overexpression vector F1007 or empty vector control, following treatment with 17-AAG. IC50 values were measured as the drug concentration required to inhibit cell growth by 50% compared with control cells (n = 3, ±SD). Increased expression of AHA1 had no significant effect on cellular sensitivity to 17-AAG (ANOVA, P > 0.05 in all cases).