Table 3.

Prediction and recognition of MiHA epitopes

Clone typeMiHA nameSNPAmino acid polymorphism*RestrictionPredicted HLA binding peptides*Measured EC50 peptide recognition (nmol/L)
H 1LB-WNK1-1Irs12828016Ile/MetA*0201RTLSPEIITV4
TLSPEIITV>5,000
H 2LB-SSR1-1Srs10004Ser/LeuA*0201SLAVAQDLT>5,000
H 3LB-PRCP-1Drs2298668Asp/GluA*0201FMWDVAEDLKA1
FMWDVAEDL17
H 8LB-ERAP1-1Rrs26653Arg/ProB*0702HPRQEQIALLA20
HPRQEQIALLnt
HPRQEQIALnt
H 9LB-BCAT2-1Rrs11548193Arg/ThrB*0702QPRRALLFVIL6
QPRRALLFVI195
QPRRALLFV>5,000
GVSQPRRALL>5,000
GVSQPRRALnt
H 10LB-ARHGDIB-1Rrs4703Arg/ProB*0702LPRACWREA4
LPRACWREAR45
LPRACWREART249
H 11LB-PDCD11-1Frs2986014Phe/LeuB*0702GPDSSKTFLCL28
GPDSSKTFL>5,000
Z 1LB-GEMIN4-1Vrs4968104Val/GluB*0702FPALRFVEV3
Z 2LB-EBI3-1Irs4740Ile/ValB*0702RPRARYYIQVA480
RPRARYYIQV13
RPRARYYIQnt
AVRPRARYYInt
Z 3LB-APOBEC3B-1Krs2076109Lys/GluB*0702KPQYHAEMCFL4,447
KPQYHAEMCF285
KPQYHAEMC1,604

Abbreviation: nt, not tested.

  • *Patient-type amino acid residues are underlined.

  • Amino acid polymorphism after translation in an ARF.