Table 1.

Production of PGE2 by melanoma cell lines cultured in the absence and in the presence of IFNγ or cocultured with NK cells (in the presence or in the absence of the PGE2 inhibitor NS-398)

Melanoma cell lineAlonePlus IFNγaPlus NK cellsBPlus NK cells plus NS-398c
MeCoPNDdND48 ± 1867.5 ± 52.5
MeMO2,914 ± 1,25310,876 ± 3,10412,650 ± 919.227.5 ± 17.7
MePA511 ± 140592 ± 1924,127 ± 282ND

Data refer to the levels of PGE2 expressed at pg/mL. Results are the means ± SD of 3 independent experiments.

  • aThe levels of PGE2 were evaluated after 6 days of culture in supernatants derived from melanoma cells stimulated with IFNγ.

  • bThe levels of PGE2 were evaluated after 6 days of culture in supernatants derived from melanoma cells cocultured with NK cells.

  • cThe levels of PGE2 were evaluated after 6 days of culture in supernatants derived from melanoma cells cocultured with NK cells in the presence of the PGE2 inhibitor NS-398.

  • dND, not detectable.