Abstract
Murine monoclonal hybridoma antibodies were generated efficiently by using a human leukemia antigen preparation which was isolated from the cell membranes of MOLT-4 (a T-leukemia cell line) by means of a novel system. The generated monoclonal hybridoma antibodies were screened and characterized by a radioimmunoassay using a variety of cell specimens as targets. The results showed that the antigen(s) defined by several of these monoclonal hybridoma antibodies is associated with human T-cell leukemia and further suggest that this antigen(s) is a new type of human leukemia-associated cell surface antigen.
Footnotes
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↵1 This investigation was supported by USPHS Grant CA19304 awarded by the NIH, Department of Health, Education, and Welfare.
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↵2 To whom requests for reprints should be addressed.
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↵3 A unit of the N. Y. State Department of Health.
- Received February 25, 1982.
- Accepted July 13, 1982.
- ©1982 American Association for Cancer Research.
Volume 42, Issue 10
