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Tumor Progression in Four Mammary Epithelial Cell Lines Derived from the Same Patient

Vimla Band, Deborah Zajchowski, Karen Swisshelm, Douglas Trask, Victoria Kulesa, Craig Cohen, James Connolly and Ruth Sager
Vimla Band
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Deborah Zajchowski
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Karen Swisshelm
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Douglas Trask
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Victoria Kulesa
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Craig Cohen
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James Connolly
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Ruth Sager
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DOI:  Published November 1990
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Abstract

Two primary and two metastatic cell lines with distinct phenotypes and genotypes have been established from a patient diagnosed as having infiltrating and intraductal mammary carcinoma (21T series). All four lines can be cultured in the same medium, DFCI-1, which also supports long-term growth of normal epithelial cells. Therefore, we have been able to compare normal and tumor cells at the cellular and molecular levels. The mammary origin of the 21T series was confirmed by using antibodies against the human milk fat globule antigen-2 epitope. The two primary tumor lines (21NT and 21PT) are both immortal and aneuploid, although only 21NT is tumorigenic in the nude mouse assay. The two populations derived from the metastatic pleural effusion (21MT-1 and 21MT-2) each exhibit distinct characteristics in morphology and growth factor requirements. The erbB2 gene is amplified and overexpressed in all of these cell lines compared to normal epithelial cell controls. These four tumor cell lines from a single patient represent a mammary tumor progression series that has been established in long-term cell culture.

Footnotes

  • ↵1 This work was supported by NIH Grant CA39814.

  • Received April 27, 1990.
  • Accepted August 15, 1990.
  • ©1990 American Association for Cancer Research.
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November 1990
Volume 50, Issue 22
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Tumor Progression in Four Mammary Epithelial Cell Lines Derived from the Same Patient
Vimla Band, Deborah Zajchowski, Karen Swisshelm, Douglas Trask, Victoria Kulesa, Craig Cohen, James Connolly and Ruth Sager
Cancer Res November 15 1990 (50) (22) 7351-7357;

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Tumor Progression in Four Mammary Epithelial Cell Lines Derived from the Same Patient
Vimla Band, Deborah Zajchowski, Karen Swisshelm, Douglas Trask, Victoria Kulesa, Craig Cohen, James Connolly and Ruth Sager
Cancer Res November 15 1990 (50) (22) 7351-7357;
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