Abstract
Frequent allelic deletions in tumor cells are indicative of the inactivation of tumor suppressor genes. Recently, we isolated the single-copy sequence del-27 (I. Wieland, M. Böhm, and S. Bogatz, Proc. Natl. Acad. Sci. USA, 89: 9705–9709, 1992). Here we show that del-27 detects a restriction fragment length polymorphism that allows examination for loss of heterozygosity (LOH) in tumor specimens. LOH at the del-27 locus occurred in 57% (4 of 7) of the informative lung carcinomas independent of the histopathological differentiation grade. LOH for exon 11 of the APC gene occurred in 71% (5 of 7) of the informative cases but was not associated with LOH at the del-27 locus. The del-27 sequence was localized to chromosome 5p13–5q14, proximal to the MCC/APC region, using a somatic cell hybrid panel. Together with our previous finding that del-27 is deleted homozygously in a lung carcinoma cell line, these results suggest that del-27 is linked closely to a novel putative tumor suppressor gene.
Footnotes
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↵1 This work was supported by the Deutsche Forschungsgemeinschaft (SFB 354) and by the Bennigsen-Foerder-Preis to I. W.
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↵2 Recipient of a Deutsche Forschungsgemeinschaft-Habilitationsstipendium. To whom requests for reprints should be addressed.
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↵3 Urologische Klinik, Universitätsklinikum, Hufelandstrasse 55, 45122 Essen, Germany.
- Received September 20, 1993.
- Accepted January 25, 1994.
- ©1994 American Association for Cancer Research.
Volume 54, Issue 7
