Abstract
The use of blochemical fractionation, immunofluorescence laser-scanning confocal microscopy, and immunoelectron microscopy with mouse anti-human bcl-2 monoclonal antibody to analyze the subcellular localization of the bcl-2 gene product revealed the protein prominently in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial membranes. Electron microscopy at high magnification more precisely localized bcl-2 to the nuclear outer membrane as confirmed by the biochemical fractionation, as well as to mitochondrial outer and, to a lesser degree, inner membrane. This multisite membrane distribution of bcl-2 suggests an important role for this protein in several different membrane compartments.
Footnotes
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↵1 This work was supported by a grant-in-aid for science research from the Ministry of Education, Science, and Culture, and for the comprehensive 10-Year Strategy for Cancer Control from the Ministry of Health and Welfare.
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↵2 To whom requests for reprints should be addressed.
- Received April 8, 1993.
- Accepted February 28, 1994.
- ©1994 American Association for Cancer Research.
Volume 54, Issue 9
