Abstract
A range of DNA-damaging agents has been shown to increase cellular levels of the nuclear phosphoprotein p53 and to induce p53-dependent processes. We examined the ability of three microtubule-active agents, taxol, vinblastine, and nocodazole, to increase p53 levels and activate p53-dependent processes. When tested using a p53 DNA-binding assay, all three agents induced p53 in a dose-dependent manner. To varying degrees, these agents also induced p21WAF1/CIP1 mRNA and transcription in a chloramphenicol acetyl transferase reporter system. These data suggest there is an additional pathway for activating p53 and subsequent p53-dependent processes.
Footnotes
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↵1 Supported by funds from the Joint Center for Radiation Therapy.
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↵2 To whom requests for reprints should be addressed, at Joint Center for Radiation Therapy, 50 Binney Street, Boston, MA 02115.
- Received July 24, 1995.
- Accepted November 1, 1995.
- ©1995 American Association for Cancer Research.
Volume 55, Issue 24
