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Advances in Brief

Hypermethylation of the p16 Gene in Nasopharyngeal Carcinoma

Kwok-Wai Lo, Siu-Tim Cheung, Sing-Fai Leung, Andrew van Hasselt, Yuen-Shan Tsang, Ko-Fung Mak, Yuk-Fei Chung, John K. S. Woo, Joseph C. K. Lee and Dolly P. Huang
Kwok-Wai Lo
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Siu-Tim Cheung
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Sing-Fai Leung
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Andrew van Hasselt
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Yuen-Shan Tsang
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Ko-Fung Mak
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Yuk-Fei Chung
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John K. S. Woo
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Joseph C. K. Lee
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Dolly P. Huang
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DOI:  Published June 1996
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Abstract

We have recently reported that inactivation of the p16 gene by mutation and deletion is common in nasopharyngeal carcinoma (NPC). The present study demonstrates that hypermethylation of the 5′ CpG island can serve as an alternative mechanism for inactivation of the p16 gene in this tumor. Using Southern blotting analysis and multiplex PCR, aberrant methylation of the 5′ CpG island of the p16 gene was found in a NPC xenograft (xeno-666) and 6 (22%) of 27 primary tumors, but not in normal tissues of the nasopharynx. In the NPC xenograft (xeno-666) and its newly derived cell line (cell-666), both showing hypermethylation of the p16 gene, no p16 gene expression was found. After treatment with 5-aza-2′-deoxycytidine, reexpression of the p16 gene was detected in the cell line cell-666. These findings suggest that aberrant methylation of the 5′ CpG island may participate in the transcriptional inactivation of the p16 gene in NPC. The present results further support that the p16 gene is the critical target on chromosome 9p21 for inactivation during the development of this disease.

Footnotes

  • ↵1 Supported by a research grant (Hong Kong Grant CUHK52/93M) from the University and Polytechnic Grants Committee, Hong Kong.

  • ↵2 To whom requests for reprints should be addressed. Phone: (852) 2632-1136; Fax: (852) 2637-6274.

  • Received March 25, 1996.
  • Accepted April 30, 1996.
  • ©1996 American Association for Cancer Research.
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June 1996
Volume 56, Issue 12
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Hypermethylation of the p16 Gene in Nasopharyngeal Carcinoma
Kwok-Wai Lo, Siu-Tim Cheung, Sing-Fai Leung, Andrew van Hasselt, Yuen-Shan Tsang, Ko-Fung Mak, Yuk-Fei Chung, John K. S. Woo, Joseph C. K. Lee and Dolly P. Huang
Cancer Res June 15 1996 (56) (12) 2721-2725;

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Hypermethylation of the p16 Gene in Nasopharyngeal Carcinoma
Kwok-Wai Lo, Siu-Tim Cheung, Sing-Fai Leung, Andrew van Hasselt, Yuen-Shan Tsang, Ko-Fung Mak, Yuk-Fei Chung, John K. S. Woo, Joseph C. K. Lee and Dolly P. Huang
Cancer Res June 15 1996 (56) (12) 2721-2725;
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