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Advances in Brief

Hybrid Selection of Transcribed Sequences from Microdissected DNA: Isolation of Genes within an Amplified Region at 20q11–q13.2 in Breast Cancer

Xin-Yuan Guan, Jun Xu, Sarah L. Anzick, Hongen Zhang, Jeffrey M. Trent and Paul S. Meltzer
Xin-Yuan Guan
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Jun Xu
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Sarah L. Anzick
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Hongen Zhang
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Jeffrey M. Trent
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Paul S. Meltzer
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DOI:  Published August 1996
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Abstract

In human breast carcinomas, increased copy number of DNA sequences derived from the long arm of chromosome 20 (20q) has been commonly observed by both chromsome microdissection and comparative genomic hybridization. This chromosomal region is likely to contain one or more genes that are the biological targets of this amplification event. We describe here the utilization of a chromosome microdissection-hybrid selection strategy to isolate transcribed sequences from microdisected homogenously staining regions encompassing 20q. Using this strategy, we have isolated three novel amplified genes (termed AIBI, AIB3, and AIB4) from a cDNA library constructed from the 20q amplified breast cancer cell line BT-474. These three genes were mapped to 20q11 (AIB3 and AIB4 and 20q12 (AIB1) by fluorescence in situ hybridization. Our results indicate an unsuspected complexity to the amplification pattern of 20q in breast cancer and provide probes that will be useful for further characterization of tumor specimens.

Footnotes

  • ↵1 To whom requests for reprints should be addressed, at Laboratory of Cancer Genetics, National Center for Human Genome Research, NIH, 9000 Rockville Pike, Building 49, Room 4A22, Bethesda, MD 20892-4470. Phone: (301) 594-5283; Fax: (301) 402-3241.

  • Received May 3, 1996.
  • Accepted June 23, 1996.
  • ©1996 American Association for Cancer Research.
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August 1996
Volume 56, Issue 15
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Hybrid Selection of Transcribed Sequences from Microdissected DNA: Isolation of Genes within an Amplified Region at 20q11–q13.2 in Breast Cancer
Xin-Yuan Guan, Jun Xu, Sarah L. Anzick, Hongen Zhang, Jeffrey M. Trent and Paul S. Meltzer
Cancer Res August 1 1996 (56) (15) 3446-3450;

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Hybrid Selection of Transcribed Sequences from Microdissected DNA: Isolation of Genes within an Amplified Region at 20q11–q13.2 in Breast Cancer
Xin-Yuan Guan, Jun Xu, Sarah L. Anzick, Hongen Zhang, Jeffrey M. Trent and Paul S. Meltzer
Cancer Res August 1 1996 (56) (15) 3446-3450;
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