Abstract
By targeting the ATP binding conserved domain in three ATP binding cassette superfamily proteins (P-glycoprotein, multidrug resistance protein, and cystic fibrosis transmembrane regulator), we isolated the cDNA of a new ATP binding cassette superfamily that was specifically enhanced in a cisplatin-resistant human head and neck cancer KB cell line. A human clone homologous to rat canalicular multispecific organic anion transporter (cMOAT) was found and designated human cMOAT. Fluorescence in situ hybridization demonstrated the chromosomal locus of the gene on chromosome 10q24. The human cMOAT cDNA hybridized a 6.5-kb mRNA that was expressed 4- to 6-fold higher by three cisplatin-resistant cell lines derived from various human tumors exhibiting decreased drug accumulation. Human cMOAT may function as a cellular cisplatin transporter.
Footnotes
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↵1 Supported by a grant-in-aid for cancer research from the Ministry of Education, Science, Sports and Culture, Japan; the Fukuoka Anticancer Research Fund, Fukuoka 21st Century Medical Fund; and the Yasuda Memorial Medical Grant for Cancer Research. The nucleotide sequence reported in this paper has been deposited in the Genbank data base (Accession No. U63970).
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↵2 To whom requests for reprints should be addressed, at Department of Biochemistry, Kyushu University School of Medicine, Maidashi, Fukuoka 812-82, Japan. Fax: 81-92-632-4198; E-mail: kuwano@biocheml.med.kyushu-u.ac.jp.
- Received June 4, 1996.
- Accepted July 31, 1996.
- ©1996 American Association for Cancer Research.
Volume 56, Issue 18
