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Identification of a Breast Cancer-specific Gene, BCSG1, by Direct Differential cDNA Sequencing

Hongjun Ji, Yiliang E. Liu, Tongli Jia, Mingsheng Wang, Jingwen Liu, Guowei Xiao, Benjamin K. Joseph, Craig Rosen and Y. Eric Shi
Hongjun Ji
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Yiliang E. Liu
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Tongli Jia
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Mingsheng Wang
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Jingwen Liu
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Guowei Xiao
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Benjamin K. Joseph
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Craig Rosen
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Y. Eric Shi
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DOI:  Published February 1997
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Abstract

A high-throughput direct-differential cDNA sequencing approach was employed to identify genes differentially expressed in normal breast as compared with breast cancer. Approximately 6000 expressed sequence tags (ESTs) from cDNA libraries of normal breast and breast carcinoma were selected randomly and subjected to EST-sequencing analysis. The relative expression levels of more than 2000 unique EST groups were quantitatively compared in normal versus cancerous breast. Of many putative differentially expressed genes, a breast cancer-specific gene, BCSG1, which was expressed in high abundance in a breast cancer cDNA library but scarcely in a normal breast cDNA library, was identified as a putative breast cancer marker. In situ hybridization analysis demonstrated stage-specific BCSG1 expression as follows: BCSG1 was undetectable in normal or benign breast lesions, showed partial expression in ductal carcinoma in situ, but was expressed at an extremely high level in advanced infiltrating breast cancer. The predicted amino acid sequence of BCSG1 gene has a significant sequence homology to the non-amyloid β protein fragment of the Alzheimer's disease amyloid protein. BCSG1 overexpression may indicate breast cancer malignant progression from benign breast or in situ carcinoma to the highly infiltrating carcinoma.

Footnotes

  • ↵1 Supported in part by grants from US Army Breast Cancer Research Program (DAMD17-94-J-4149) and Helen and Irving Schneider.

  • ↵2 To whom requests for reprints should be addressed, at Pediatric Research Center, Schneider Children's Hospital, Long Island Jewish Medical Center, Albert Einstein College of Medicine, New Hyde Park, NY 11042. Phone: (718) 470-3086; Fax: (718) 470-6744; E-mail: shi@aecom.yu.edu.

  • Received August 22, 1996.
  • Accepted December 20, 1996.
  • ©1997 American Association for Cancer Research.
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February 1997
Volume 57, Issue 4
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Identification of a Breast Cancer-specific Gene, BCSG1, by Direct Differential cDNA Sequencing
Hongjun Ji, Yiliang E. Liu, Tongli Jia, Mingsheng Wang, Jingwen Liu, Guowei Xiao, Benjamin K. Joseph, Craig Rosen and Y. Eric Shi
Cancer Res February 15 1997 (57) (4) 759-764;

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Identification of a Breast Cancer-specific Gene, BCSG1, by Direct Differential cDNA Sequencing
Hongjun Ji, Yiliang E. Liu, Tongli Jia, Mingsheng Wang, Jingwen Liu, Guowei Xiao, Benjamin K. Joseph, Craig Rosen and Y. Eric Shi
Cancer Res February 15 1997 (57) (4) 759-764;
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