Skip to main content
  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

AACR logo

  • Register
  • Log in
  • Log out
  • My Cart
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

  • AACR Publications
    • Blood Cancer Discovery
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in
  • Log out
  • My Cart

Search

  • Advanced search
Cancer Research
Cancer Research
  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Meeting Abstracts
    • Collections
      • COVID-19 & Cancer Resource Center
      • Focus on Computer Resources
      • Highly Cited Collection
      • Editors' Picks
      • "Best of" Collection
  • For Authors
    • Information for Authors
    • Author Services
    • Early Career Award
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • Editors' Picks
    • OnlineFirst
    • Citations
    • Author/Keyword
    • RSS Feeds
    • My Alert Summary & Preferences
  • News
    • Cancer Discovery News
  • COVID-19
  • Webinars
  • Search More

    Advanced Search

Advances in Brief

Adenoviral Transgene Expression of MMAC/PTEN in Human Glioma Cells Inhibits Akt Activation and Induces Anoikis

Michael A. Davies, Yiling Lu, Tetsuro Sano, Xianjun Fang, Ping Tang, Ruth LaPushin, Dimpy Koul, Robert Bookstein, David Stokoe, W. K. Alfred Yung, Gordon B. Mills and Peter A. Steck
Michael A. Davies
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yiling Lu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsuro Sano
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xianjun Fang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ping Tang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruth LaPushin
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dimpy Koul
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert Bookstein
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David Stokoe
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. K. Alfred Yung
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gordon B. Mills
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter A. Steck
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published December 1998
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

The MMAC/PTEN tumor suppressor gene encodes for a phosphatase that recently has been shown to have phosphotidylinositol phosphatase activity, implicating its possible involvement in phosphatidylinositol 3′-kinase-mediated signaling. To investigate possible alterations in growth factor-mediated signal transduction, an adenovirus containing MMAC/PTEN, Ad-MMAC, previously shown to inhibit growth and tumorigenicity in glioma cells, was used to acutely express the transgene. Human glioma cells infected with Ad-MMAC but not with control adenoviruses exhibited an inhibition of phosphorylation of both activating residues of Akt, Ser-473, and Thr-308, along with Akt's serine/threonine kinase activity, without significantly altering Akt expression. The effects of functional MMAC/PTEN expression were relatively specific, because members of several other growth factor-mediated signaling pathways showed no altered responses. The presence of MMAC/PTEN also inhibited phosphorylation of BAD, although no evidence of apoptosis in the in situ treated cells was observed. However, U251 glioma cells infected with Ad-MMAC were induced to undergo anoikis at a significantly higher rate than U251 cells treated with control viruses or mock infected with media. These results demonstrate that the acute administration of MMAC/PTEN results in the inhibition of Akt-mediated signaling, growth inhibition, and anoikis, implying that loss of MMAC/PTEN increases cellular proliferation and significantly augments a cell's survival potential during cellular processes that are associated with malignancy.

Footnotes

  • ↵1 Supported by NIH Grants CA56041 and CA55261, State of Texas Advanced Technology Program Grant 97-110, and the Pediatric Brain Tumor Foundation (to P. A. S.) and NIH Grant CA71418 (to G. B. M.).

  • ↵2 The first two authors contributed equally to this work.

  • ↵3 To whom requests for reprints should be addressed, at Department of Neuro-Oncology, The University of Texas M. D. Anderson Cancer Center, Box 316, 1515 Holcombe Boulevard, Houston, TX 77030. Phone: (713) 792-3003; Fax: (713) 745-1183; E-mail: steckpa@audumla.mdacc.tmc.edu.

  • Received August 20, 1998.
  • Accepted October 16, 1998.
  • ©1998 American Association for Cancer Research.
PreviousNext
Back to top
December 1998
Volume 58, Issue 23
  • Table of Contents
  • Table of Contents (PDF)
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)

Sign up for alerts

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Adenoviral Transgene Expression of MMAC/PTEN in Human Glioma Cells Inhibits Akt Activation and Induces Anoikis
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Adenoviral Transgene Expression of MMAC/PTEN in Human Glioma Cells Inhibits Akt Activation and Induces Anoikis
Michael A. Davies, Yiling Lu, Tetsuro Sano, Xianjun Fang, Ping Tang, Ruth LaPushin, Dimpy Koul, Robert Bookstein, David Stokoe, W. K. Alfred Yung, Gordon B. Mills and Peter A. Steck
Cancer Res December 1 1998 (58) (23) 5285-5290;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Adenoviral Transgene Expression of MMAC/PTEN in Human Glioma Cells Inhibits Akt Activation and Induces Anoikis
Michael A. Davies, Yiling Lu, Tetsuro Sano, Xianjun Fang, Ping Tang, Ruth LaPushin, Dimpy Koul, Robert Bookstein, David Stokoe, W. K. Alfred Yung, Gordon B. Mills and Peter A. Steck
Cancer Res December 1 1998 (58) (23) 5285-5290;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

Advances in Brief

  • NIMA-Related Protein Kinase 1 Is Involved Early in the Ionizing Radiation-Induced DNA Damage Response
  • Conditional Expression of K-ras in an Epithelial Compartment that Includes the Stem Cells Is Sufficient to Promote Squamous Cell Carcinogenesis
  • Crucial Role of Phospholipase Cε in Chemical Carcinogen-Induced Skin Tumor Development
Show more Advances in Brief

Articles

  • BRCA1, BRCA2, and Rad51 Operate in a Common DNA Damage Response Pathway
  • Introduction of H. Robert Horvitz
  • Membership of Selection Committees
Show more Articles
  • Home
  • Alerts
  • Feedback
  • Privacy Policy
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2021 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement